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首页> 外文期刊>Electroanalysis >Enhancing a Novel Robust Multicomposite Materials Platform for Glucose Biosensors
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Enhancing a Novel Robust Multicomposite Materials Platform for Glucose Biosensors

机译：增强一种用于葡萄糖生物传感器的新型鲁棒多重材料平台

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An effective, stable enzymatic glucose biosensor was fabricated on a glassy carbon electrode (GCE) surface using simple multicomposite materials (MCM): a solution of prepared poly(diallyldimethylammonium chloride)-capped gold nanoparticles-nickel ferrite particles-carbon nanotubes-chitosan (PDDA-AuNPs-NiFe2O4-CNTs-CHIT), electropolymerization of poly(o-phenylenediamine) (PoPD) and immobilization of glucose oxidase (GOx). Biocompatibility and synergy of the MCM enhanced the immobilization and the reaction of GOx and as well as the electron transfer from an oxidation reaction of hydrogen peroxide in the system. The NiFe2O4 was synthesized by co-precipitation and calcined at 700 degrees C. Characterization was carried out by field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray spectroscopy (EDX), Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD) which presented both tetrahedral and octahedral metal stretching with a cubic NiFe2O4 crystal phase. The GOx/PoPD/MCM/GCE yielded a 0.77 s(-1) charge transfer rate constant (K-s), a 2.28x10(-6) cm(2) s(-1) diffusion coefficient value (D), a 0.21 mm(2) electroactive surface area (A(e)) and a 1.93x10(-8) mol cm(-2) surface concentration (gamma) as determined by cyclic voltammetry. The modified electrode showed a durable operation time (n=97, more than 50 % I), repeatability (%RSD=0.38, n=10), reproducibility (%RSD=1.60, n=10), high sensitivity (853.07 mu A mM(-1) cm(-2)), selectivity without effects of electroactive species (aspirin, uric acid, caffeine, cholesterol, ascorbic acid and dopamine) and two linear ranges from 0.5 to 10 mu M (R-2=0.998) and 10 to 15,000 mu M (R-2=0.991) with a low detection limit (0.35 mu M, S/N=3). Its Michaelis-Menten constant (K-m) was calculated as 93.51 mu M with 46.30 mu A maximum current (I-max). This proposed simple method was successfully applied for glucose determination in human blood samples.
机译：使用简单的多重材料（MCM）在玻璃电极（GCE）表面上制造有效的稳定的酶促糖生物传感器：制备的聚（二烯丙基甲基氯化铵） - 封装的金纳米颗粒 - 镍铁氧体粒子 - 碳纳米管 - 壳聚糖（PDDA）溶液（PDDA -aUnps-niFe2O4-cnts-chit），聚（o-苯二胺）（popd）的电聚合和葡萄糖氧化酶（GOX）的固定化。 MCM的生物相容性和协同作用增强了GOX的固定化和反应，以及来自系统中过氧化氢的氧化反应的电子转移。通过共沉淀合成NiFe2O4并在700℃下煅烧。表征通过现场发射扫描电子显微镜（Fe-SEM），能量分散X射线光谱（EDX），傅里叶变换红外光谱（FTIR）和X射线衍射（XRD）用立方体NiFe2O4晶相拉伸展示四面体和八面体金属。 GOX / POPD / MCM / GCE产生0.77秒（-1）电荷转移速率常数（Ks），2.28x10（-6）cm（2）S（-1）扩散系数值（d），0.21 mm （2）通过循环伏安法测定的（2）电活性表面积（A（e））和1.93×10（-8）摩尔Cm（-2）表面浓度（γ）。修饰的电极显示耐用的操作时间（n = 97，超过50％i），重复性（％RSD = 0.38，n = 10），再现性（％RSD = 1.60，n = 10），灵敏度高（853.07亩mm（-1）cm（-2）），无需电活性物种（阿司匹林，尿酸，咖啡因，胆固醇，抗坏血酸和多巴胺）的选择性，两个线性范围为0.5至10μm（r-2 = 0.998）和10至15,000μm（r-2 = 0.991），检测极限低（0.35μm，s / n = 3）。其Michaelis-Menten常数（K-M）计算为93.51μm，最大电流为46.30亩（I-Max）。该提出的简单方法已成功应用于人血样中的葡萄糖测定。

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