Multi‐walled Carbon Nanotubes Non‐covalently Functionalized with Polyarginine: A New Alternative for the Construction of Reagentless NAD        <sup >++        /Dehydrogenase‐based Ethanol Biosensor

Gallay Pablo; Eguílaz Marcos; Rivas Gustavo

摘要

Abstract > This work reports for the first time the development of a reagentless enzymatic amperometric biosensor for ethanol based on the use of a glassy carbon electrode (GCE) modified with multi‐walled carbon nanotubes (MWCNTs) non‐covalently functionalized with polyarginine (Polyarg) as platform for the robust immobilization of alcohol dehydrogenase (ADH) and NAD + . The new strategy allows to obtain an integrated GCE/MWCNTs‐Polyarg/NAD + ‐ADH ethanol biosensor with important advantages compared to the existing ethanol biosensors: avoids the external addition of the cofactor for each measurement, ensures a fast and sensitive quantification of ethanol due to the intimate interaction of the components, and allows the detection at considerably lower potentials due to the catalytic activity of the carbon nanostructures. These unique properties have made possible a very efficient ethanol quantification with a sensitivity of (1487±6)?μA?M ?1 , detection limit of 0.65?μM, response time of 8?s, and reproducibility of 5.5?% with a very successful application for the quantification of ethanol in different commercial beverages. </abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> <div class="translation abstracttxt"> <span class="zhankaihshouqi fivelineshidden" id="abstract"> <span>机译：</span><Abstract Type =“Main”XML：Lang =“en”> <标题类型=“main”>抽象</ title> >本作工作报告首次基于乙醇的无可酶酶压生物传感器的开发使用用多壁碳纳米管（MWCNT）改性的玻碳电极（GCE）与Polyarninine（Polyarg）非共价官能团作为醇脱氢酶（ADH）和NAD <SUP> + </ SUP的鲁棒固定>。与现有的乙醇生物传感器相比，新策略允许获得集成的GCE / MWCNTS-Polyarg / NAD + </ sup> -adh乙醇生物传感器的重要优点：避免对每个测量的外部添加辅因子，确保a由于组分的抗体相互作用，乙醇的快速和敏感量化，并且由于碳纳米结构的催化活性，允许在显着降低电位下检测。这些独特的性能使得具有（1487±6）ωμAΩ·m α1</ sup>，0.65Ωμm，响应时间为8？s的响应时间和再现性的极高的乙醇定量5.5？％，非常成功地申请量化不同商业饮料中乙醇。 </ p> </ abstract> </span> <span class="z_kbtn z_kbtnclass hoverxs" style="display: none;">展开▼</span> </div> </div> <div class="record"> <h2 class="all_title" id="enpatent33" >著录项</h2> <ul> <li> <span class="lefttit">来源</span> <div style="width: 86%;vertical-align: text-top;display: inline-block;"> <a href='/journal-foreign-22546/'>《Electroanalysis》</a> <b style="margin: 0 2px;">|</b><span>2019年第5期</span><b style="margin: 0 2px;">|</b><span>共8页</span> </div> </li> <li> <div class="author"> <span class="lefttit">作者</span> <p id="fAuthorthree" class="threelineshidden zhankaihshouqi"> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gallay Pablo&option=202" target="_blank" rel="nofollow">Gallay Pablo;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Eguílaz Marcos&option=202" target="_blank" rel="nofollow">Eguílaz Marcos;</a> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Rivas Gustavo&option=202" target="_blank" rel="nofollow">Rivas Gustavo;</a> </p> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zkzz" style="display: none;">展开▼</span> </div> </li> <li> <div style="display: flex;"> <span class="lefttit">作者单位</span> <div style="position: relative;margin-left: 3px;max-width: 639px;"> <div class="threelineshidden zhankaihshouqi" id="fOrgthree"> <p>INFIQC Departamento de Fisicoquímica Facultad de Ciencias QuímicasCiudad Universitaria5000 Córdoba Argentina;</p> <p>INFIQC Departamento de Fisicoquímica Facultad de Ciencias QuímicasCiudad Universitaria5000 Córdoba Argentina;</p> <p>INFIQC Departamento de Fisicoquímica Facultad de Ciencias QuímicasCiudad Universitaria5000 Córdoba Argentina;</p> </div> <span class="z_kbtnclass z_kbtnclassall hoverxs" id="zhdw" style="display: none;">展开▼</span> </div> </div> </li> <li > <span class="lefttit">收录信息</span> <span style="width: 86%;vertical-align: text-top;display: inline-block;"></span> </li> <li> <span class="lefttit">原文格式</span> <span>PDF</span> </li> <li> <span class="lefttit">正文语种</span> <span>eng</span> </li> <li> <span class="lefttit">中图分类</span> <span><a href="https://www.zhangqiaokeyan.com/clc/4817.html" title="电化学、电解、磁化学">电化学、电解、磁化学;</a></span> </li> <li class="antistop"> <span class="lefttit">关键词</span> <p style="width: 86%;vertical-align: text-top;"> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Multi-walled carbon nanotubes&option=203" rel="nofollow">Multi-walled carbon nanotubes;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Ethanol amperometric biosensor&option=203" rel="nofollow">Ethanol amperometric biosensor;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=NADH&option=203" rel="nofollow">NADH;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Polyarginine&option=203" rel="nofollow">Polyarginine;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=NAD + immobilization&option=203" rel="nofollow">NAD + immobilization;</a> <a style="color: #3E7FEB;" href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Reagentless biosensor.&option=203" rel="nofollow">Reagentless biosensor.;</a> </p> <div class="translation"> 机译：多壁碳纳米管;乙醇安培菌生物传感器;NADH;polyarninine;NAD +固定化;无毒的生物传感器。; </div> </li> </ul> </div> </div> <div class="literature cardcommon"> <div class="similarity "> <h3 class="all_title" id="enpatent66">相似文献</h3> <div class="similaritytab clearfix"> <ul> <li class="active" >外文文献</li> <li >中文文献</li> <li >专利</li> </ul> </div> <div class="similarity_details"> <ul > <li> <div> <b>1. </b><a class="enjiyixqcontent" href="/journal-foreign-detail/0704023174967.html">Multi‐walled Carbon Nanotubes Non‐covalently Functionalized with Polyarginine: A New Alternative for the Construction of Reagentless NAD <sup >++ /Dehydrogenase‐based Ethanol Biosensor</a> <b>[J]</b> . <span> <a href="/search.html?doctypes=4_5_6_1-0_4-0_1_2_3_7_9&sertext=Gallay 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Multi‐walled Carbon Nanotubes Non‐covalently Functionalized with Polyarginine: A New Alternative for the Construction of Reagentless NAD ++ /Dehydrogenase‐based Ethanol Biosensor

摘要

Multi‐walled Carbon Nanotubes Non‐covalently Functionalized with Polyarginine: A New Alternative for the Construction of Reagentless NAD ^{++ /Dehydrogenase‐based Ethanol Biosensor}