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首页> 外文期刊>BJU international >Acute urinary retention and subsequent catheterization cause lipid peroxidation and oxidative DNA damage in the bladder: preventive effect of edaravone, a free-radical scavenger.
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Acute urinary retention and subsequent catheterization cause lipid peroxidation and oxidative DNA damage in the bladder: preventive effect of edaravone, a free-radical scavenger.

机译：急性尿retention留和随后的导管插入术会在膀胱中引起脂质过氧化和DNA氧化损伤：依达拉奉（一种自由基清除剂）的预防作用。

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OBJECTIVE: To investigate the effect of a free-radical scavenger, edaravone, on the changes occurring with acute urinary retention (AUR) and subsequent catheterization in the rat bladder. MATERIALS AND METHODS: Eight-week-old male Sprague Dawley rats were allocated to one of four groups; an AUR group that had urinary retention induced, with subsequent catheterization; two edaravone groups, given edaravone at 1 or 10 mg/kg body weight for 60 min and then the same urinary retention and subsequent catheterization; and a sham-operated control group given edaravone 10 mg/kg. Urinary retention was induced by the clamping the rat penile urethra with a small clip, making a cystostomy, and then infusing 3 mL (0.6 mL/min) of saline with an infusion pump. The obstruction was sustained for 30 min and then the bladder was allowed to drain with a catheter in place for 60 min as the studies continued. After killing the rats the function of the bladder was assessed, with carbachol and 100 mM KCl, and the levels of malondialdehyde (MDA, a marker of lipid peroxidation), 8-hydroxydeoxyguanosine (8-OHdG; a marker of oxidative DNA damage), heat-shock protein 70 (HSP 70) and its mRNA were measured. RESULTS: AUR increased the intravesical pressure and decreased blood flow, and subsequent catheterization decreased the intravesical pressure and increased blood flow. Edaravone induced a decrease in blood flow in the bladder during the urinary retention and subsequent catheterization compared to the blood flow in the AUR group. Edaravone resulted in protection of the contractile responses to both carbachol and KCl in a dose-dependent manner. The MDA concentration, 8-OHdG content and expressions of HSP-70 and its mRNA in the AUR group were significantly larger than those of the control group. Edaravone markedly suppressed the accumulations of MDA and 8-OHdG in the bladder, and reduced the expressions of HSP 70 and its mRNA. CONCLUSION: These results indicate that edaravone reduces the oxidative stress and prevents the bladder dysfunction caused by AUR and subsequent catheterization.
机译：目的：研究自由基清除剂依达拉奉对急性尿retention留（AUR）和随后在大鼠膀胱中插入导管后发生的变化的影响。材料与方法：将八周大的Sprague Dawley雄性大鼠分为四组之一。诱导尿retention留的AUR组，随后进行导管插入术;两组依达拉奉，分别以1或10 mg / kg体重给予依达拉奉60分钟，然后进行相同的尿retention留和随后的导管插入术;假手术对照组给予依达拉奉10 mg / kg。用小夹子夹住大鼠阴茎尿道，进行膀胱造口术，然后用输液泵输注3 mL（0.6 mL / min）盐水诱导尿retention留。阻塞持续30分钟，然后随着研究的进行，用适当的导管将膀胱引流60分钟。杀死大鼠后，用卡巴胆碱和100 mM KCl评估膀胱的功能，并评估丙二醛（脂质过氧化的标志物MDA），8-羟基脱氧鸟苷（8-OHdG;氧化性DNA损伤的标志物）的水平，测量了热激蛋白70（HSP 70）及其mRNA。结果：AUR增加了膀胱内压力并减少了血流量，随后的导管插入术降低了膀胱内压力并增加了血流量。与AUR组的血流量相比，依达拉奉在尿retention留和随后的导管插入期间引起膀胱血流量减少。依达拉奉以剂量依赖的方式保护了对卡巴胆碱和氯化钾的收缩反应。 AUR组MDA浓度，8-OHdG含量，HSP-70及其mRNA表达均明显高于对照组。依达拉奉显着抑制了膀胱中MDA和8-OHdG的积累，并降低了HSP 70及其mRNA的表达。结论：这些结果表明依达拉奉可减轻氧化应激并预防由AUR和随后的导管插入术引起的膀胱功能障碍。

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