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首页> 外文期刊>Environmental and molecular mutagenesis. >The TGx-28.65 Biomarker Online Application for Analysis of Transcriptomics Data to Identify DNA Damage-Inducing Chemicals in Human Cell Cultures
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The TGx-28.65 Biomarker Online Application for Analysis of Transcriptomics Data to Identify DNA Damage-Inducing Chemicals in Human Cell Cultures

机译：TGX-28.65 Biomarker在线应用程序分析转录组族数据，以鉴定人细胞培养物中的DNA损伤诱导的化学品

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摘要

The TGx-28.65 biomarker is a 65-gene expression profile generated from testing 28 model chemicals (13 that cause DNA damage and 15 that do not) in human TK6 cells. It is used to predict whether a chemical induces DNA damage or not. We expanded availability to the biomarker by developing the online TGx-28.65 biomarker application for predicting the DNA damage inducing (DDI) potential of suspect toxicants tested in p53-proficient human cells and assessing putative mode(s) of action (MOA). Applications like this that analyse gene expression data to predict the hazard potential of test chemicals hold great promise for risk assessment paradigms. The TGx-28.65 biomarker interfaces with an analytical tool to predict the probability that a test chemical can directly or indirectly induce DNA damage. User submitted in vitro microarray data are compared to the 28-chemical x 65-gene signature profile and the probability that the data fit the profile for a DDI or a non-DDI (NDDI) chemical is calculated. The results are displayed in the Results Table, which includes the classification probability and hyperlinks to view heatmaps, hierarchical clustering, and principal component analyses of user-input data in the context of the reference profile. The heatmaps and cluster plots, along with the corresponding text data files of fold changes in gene expression and Euclidean distances can be downloaded. Review of the test chemical data in relationship to the biomarker allows rapid identification of key gene alterations associated with DNA damage as well as chemicals in the reference set that produced a similar response. (C) 2017 Wiley Periodicals, Inc.
机译：TGX-28.65生物标志物是一种65-基因表达谱，从测试28模型化学物质（13，导致DNA损伤和15个，其中不具有不存在的15个）。它用于预测化学物质是否诱导DNA损伤。我们通过开发在线TGX-28.65生物标志物应用程序来预测在P53-易碎的人体细胞中测试的可疑毒物的DNA损伤（DDI）潜力和评估调用模式（MOA）来扩大对生物标志物的可用性。这样的应用，即分析基因表达数据以预测测试化学品的危险潜力对风险评估范例具有很大的承诺。 TGX-28.65生物标志物与分析工具接口，以预测测试化学品可以直接或间接诱导DNA损伤的可能性。将在体外微阵列数据提交的用户与28-化学x 65-基因特征分布进行比较，并且计算数据适合DDI或非DDI（NDDI）化学的轮廓。结果显示在结果表中，包括在参考配置文件的上下文中查看用于查看Heatmaps，分层群集和主组件分析的分类概率和超链接。可以下载热量和簇曲线以及基因表达和欧几里德距离的相应文本数据文件。审查与生物标志物的关系中的测试化学数据允许快速鉴定与DNA损伤相关的关键基因改变以及产生类似反应的参考组中的化学品。 （c）2017 Wiley期刊，Inc。

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