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首页> 外文期刊>African journal of medicine and medical sciences. >Effects of extracts of the leaves of Brysocarpus coccineus on rat liver mitochondrial membrane permeability transition (MMPT) pore.
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Effects of extracts of the leaves of Brysocarpus coccineus on rat liver mitochondrial membrane permeability transition (MMPT) pore.

机译：Brysocarpus Coccineus叶片提取物对大鼠肝线粒体膜渗透转变（MMPT）孔的影响。

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Objective:To examine the influence or the effect of the extracts of Brysocarpus coccineus leaves on the mitochondrial membrane permeability transition (MMPT)pore opening in rats with a view to establishing if any bioactive constituent of the plant could become useful in the chemotherapy of cancer. Materials and methods: The effects of extracts of the leaves of Brysocarpus coccineus, a medicinal plant with anti-tumour, anti-inflammatory and analgesic properties, were assessed on rat liver mitochondrial membrane permeability transition (MMPT) pore in the presence and absence of calcium in vitro and in vivo. Results: The results obtained show that calcium ions induced the opening of MMPT pore significantly (P<0.05) in rat liver mitochondria, while spermine inhibited calcium-induced opening of pore, indicating that the mitochondria were intact ab initio. The results further revealed the inhibitory effects of different concentrations (200, 600, 1000, 1400, and 1800mug/ ml) of the various extracts of the leaves compared with spermine. Specifically, the data revealed that chloroform and ethylacetate extracts reversed calcium-induced opening of MMPT pore in a concentration-dependent manner (74%, 79%,85%, 86%, 87%) for the chloroform extract and (36%, 37%, 59%, 71% and 83%) for the ethylacetate extract, respectively. On the contrary, pre-incubation of normal healthy mitochondria with the extracts in the absence of calcium resulted in the induction of the MMPT pore opening to varying degrees by these concentrations of the extracts. The chloroform extract induced pore opening in a concentration- dependent manner in the order 2.4,2.4,2.5,2.6 and 3.0 folds while the ethylacetate extracts induced the opening of the pore by 1.1,1.2,1.3,1.3 and 1.4 folds between 200-1800mug/ml, respectively. The results obtained using rats orally exposed to various doses of methanol extract of the leaves of B. coccineus for fourteen days showed that there was significant (p<0.05) induction of mitochondrial membrane permeability transition pore opening in the absence of calcium in a dose-dependent manner. Maximum induction of 26-fold was obtained at 200mg/kgbwt while the least dose (50mg/kgbdwt) gave 17 fold induction. Conclusion:The ability of the extracts of B. coccineus to induce MMPT pore opening in the absence of calcium in vitro and in vivo suggest that the leaves of the plant contain certain bioactive substances capable of inducing MMPT opening either in the original form or as formed biotrans derivative with eventual release of apoptotic proteins which may lead to apoptosis. The property of the extracts could be exploited for cancer chemotherapy when increased rate of apoptosis is required.
机译：目的：检查Brysocarpus Coccineus提取物对大鼠线粒体膜渗透转变（MMPT）孔隙开口的影响或效果，以建立植物的任何生物活性组分可用于癌症的化疗。材料和方法：在存在和不存在钙的情况下，评估了Brysocarpus Coccineus叶片叶片的叶子，药用植物，具有抗肿瘤，抗炎和镇痛性的特性的影响体外和体内。结果：得到的结果表明，钙离子在大鼠肝线粒体中显着（P <0.05）诱导了MMPT孔的开口，而精子抑制钙诱导的孔隙，表明线粒体是完整的AB INITIO。结果进一步揭示了与精胺相比叶片各种提取物的不同浓度（200,600,1000,1400和1800mug / ml）的抑制作用。具体地，数据显示，氯仿和乙酸乙酯以浓度依赖性方式（74％，79％，85％，86％，87％）用于氯仿提取物（36％，37）分别用于乙基乙酸提取物的％，59％，71％和83％。相反，在没有钙的情况下用萃取液预孵育正常健康线粒体，导致通过这些浓度的提取物诱导Mmpt孔开口到不同程度的变化。氯仿萃取诱导的孔口以浓度依赖于浓度的方式，在2.4,2.4,2.5,2.6和3.0倍，而乙基乙酸酯提取物在200-1800Mug之间诱导孔的开口1.1,1.2,1.3,1.3和1.4倍/ ml分别。使用口服暴露于B.Coccineus叶片的各种剂量的甲醇提取物的大鼠获得的结果表明，在没有钙的情况下，线粒体膜渗透过渡孔的显着（P <0.05）诱导 - 依赖的方式。在200mg / kgbwt获得最大诱导26折，而最小剂量（50mg / kgbdwt）得到17倍的诱导。结论：B. Coccine患者在体外没有钙的情况下诱导MMPT孔隙开口的能力表明，该植物的叶片含有能够以原始形式或形成的能够诱导MMPT开口的某些生物活性物质Biotrans衍生物最终释放凋亡蛋白，可能导致细胞凋亡。当需要增加的细胞凋亡率时，可以利用提取物的性质。

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