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首页> 外文期刊>African journal of medicine and medical sciences. >Effects of extracts of the leaves of Brysocarpus coccineus on rat liver mitochondrial membrane permeability transition (MMPT) pore.
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Effects of extracts of the leaves of Brysocarpus coccineus on rat liver mitochondrial membrane permeability transition (MMPT) pore.

机译：椰果树叶片提取物对大鼠肝线粒体膜通透性转变（MMPT）孔的影响。

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Objective:To examine the influence or the effect of the extracts of Brysocarpus coccineus leaves on the mitochondrial membrane permeability transition (MMPT)pore opening in rats with a view to establishing if any bioactive constituent of the plant could become useful in the chemotherapy of cancer. Materials and methods: The effects of extracts of the leaves of Brysocarpus coccineus, a medicinal plant with anti-tumour, anti-inflammatory and analgesic properties, were assessed on rat liver mitochondrial membrane permeability transition (MMPT) pore in the presence and absence of calcium in vitro and in vivo. Results: The results obtained show that calcium ions induced the opening of MMPT pore significantly (P<0.05) in rat liver mitochondria, while spermine inhibited calcium-induced opening of pore, indicating that the mitochondria were intact ab initio. The results further revealed the inhibitory effects of different concentrations (200, 600, 1000, 1400, and 1800mug/ ml) of the various extracts of the leaves compared with spermine. Specifically, the data revealed that chloroform and ethylacetate extracts reversed calcium-induced opening of MMPT pore in a concentration-dependent manner (74%, 79%,85%, 86%, 87%) for the chloroform extract and (36%, 37%, 59%, 71% and 83%) for the ethylacetate extract, respectively. On the contrary, pre-incubation of normal healthy mitochondria with the extracts in the absence of calcium resulted in the induction of the MMPT pore opening to varying degrees by these concentrations of the extracts. The chloroform extract induced pore opening in a concentration- dependent manner in the order 2.4,2.4,2.5,2.6 and 3.0 folds while the ethylacetate extracts induced the opening of the pore by 1.1,1.2,1.3,1.3 and 1.4 folds between 200-1800mug/ml, respectively. The results obtained using rats orally exposed to various doses of methanol extract of the leaves of B. coccineus for fourteen days showed that there was significant (p<0.05) induction of mitochondrial membrane permeability transition pore opening in the absence of calcium in a dose-dependent manner. Maximum induction of 26-fold was obtained at 200mg/kgbwt while the least dose (50mg/kgbdwt) gave 17 fold induction. Conclusion:The ability of the extracts of B. coccineus to induce MMPT pore opening in the absence of calcium in vitro and in vivo suggest that the leaves of the plant contain certain bioactive substances capable of inducing MMPT opening either in the original form or as formed biotrans derivative with eventual release of apoptotic proteins which may lead to apoptosis. The property of the extracts could be exploited for cancer chemotherapy when increased rate of apoptosis is required.
机译：目的：研究球果桑树提取物对大鼠线粒体膜通透性转变（MMPT）孔的开放的影响或作用，以期确定该植物的任何生物活性成分是否可用于癌症化疗。材料和方法：在有钙和无钙的情况下，对具有抗肿瘤，抗炎和镇痛作用的药用植物椰菜叶提取物对大鼠肝线粒体膜通透性转变（MMPT）孔的作用进行了评估。体外和体内。结果：获得的结果表明，钙离子显着诱导大鼠肝线粒体中MMPT孔的开放（P <0.05），而精胺抑制钙诱导的线粒体开放，表明线粒体从头开始是完整的。结果进一步揭示了与精胺相比，不同浓度（200、600、1000、1400和1800mug / ml）的各种叶提取物的抑制作用。具体而言，数据显示，氯仿和乙酸乙酯提取物以浓度依赖性的方式逆转了钙诱导的MMPT孔的开口（74％，79％，85％，86％，87％），而氯仿提取物（36％，37分别为乙酸乙酯提取物的1％，59％，71％和83％）。相反，在不存在钙的情况下，将正常健康的线粒体与提取物进行预温育会导致这些浓度的提取物在不同程度上诱导MMPT孔的开放。氯仿提取物以2.4、2.4、2.5、2.6和3.0倍的浓度依赖性方式引起孔的开放，而乙酸乙酯提取物以200、1800杯之间的1.1、1.2、1.3、1.3和1.4倍的方式诱导孔的开放。 / ml。使用大鼠口服暴露于不同剂量的球果双歧杆菌叶甲醇提取物14天获得的结果表明，在不存在钙的情况下，剂量显着（p <0.05）诱导线粒体膜通透性转变开孔-依赖方式。在200mg / kgbwt下获得最大26倍诱导，而最小剂量（50mg / kgbdwt）下产生17倍诱导。结论：在没有钙的情况下，球孢杆菌提取物在体外和体内均无诱导MMPT孔开放的能力，这表明该植物的叶片中含有某些能够诱导MMPT开放的生物活性物质，无论是原始形式还是原状。最终释放可能导致凋亡的凋亡蛋白的生物反式衍生物。当需要增加细胞凋亡率时，提取物的性质可用于癌症化学疗法。

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