• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Environmental microbiology >Functional characterization of the sterigmatocystin secondary metabolite gene cluster in the filamentous fungus Podospora anserina: involvement in oxidative stress response, sexual development, pigmentation and interspecific competitions
【24h】

Functional characterization of the sterigmatocystin secondary metabolite gene cluster in the filamentous fungus Podospora anserina: involvement in oxidative stress response, sexual development, pigmentation and interspecific competitions

机译：丝状真菌Podospora Anserina中的甾醇次生β次级代谢物基因簇的功能表征：涉及氧化应激反应，性发育，色素沉着和间隙竞争

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Filamentous fungi are known as prolific untapped reservoirs of diverse secondary metabolites, where genes required for their synthesis are organized in clusters. The bioactive properties of these compounds are closely related to their functions in fungal biology, which are not well understood. In this study, we focused on the Podospora anserina gene cluster responsible for the biosynthesis of sterigmatocystin (ST). Deletion of the PaStcA gene encoding the polyketide synthase and overexpression (OE) of the PaAflR gene encoding the ST-specific transcription factor in P. anserina were performed. We showed that growth of PaStcA(Delta) was inhibited in the presence of methylglyoxal, while OE-PaAflR showed a little inhibition, indicating that ST production may enhance oxidative stress tolerance in P. anserina. We also showed that the OE-PaAflR strain displayed an overpigmented thallus mediated by the melanin pathway. Overexpression of PaAflR also led to sterility. Interspecific confrontation assays showed that ST-overexpressed strains produced a high level of peroxides and possessed a higher competitiveness against other fungi. Comparative metabolite profiling demonstrated that PaStcA(Delta) strain was unable to produce ST, while OE-PaAflR displayed a ST overproduction. This study contributes to a better understanding of ST in P. anserina, especially with regard to its involvement in fungal physiology.
机译：丝状真菌被称为多种次级代谢物的多产储层，其中它们合成所需的基因被组织在簇中。这些化合物的生物活性性质与其在真菌生物学中的功能密切相关，这是不太了解的。在这项研究中，我们专注于负责甾醇（ST）生物合成的Podospora Anserina基因簇。进行编码编码P. anserina中的ST-特异性转录因子的PaaflR基因的聚酮化合酶和过表达（OE）的缺失。我们表明，在甲基甘油氧的存在下，患者的生长被抑制，而OE-Paaflr显示出一点抑制，表明ST产生可以增强P. Anserina的氧化应激耐受性。我们还表明，OE-Paaflr菌株显示出由黑色素途径介导的过度介导的晶粒。 Paaflr的过度表达也导致无菌。间隙的对抗测定表明，ST过表达菌株产生了高水平的过氧化物，并对其他真菌具有更高的竞争力。比较代谢物分析表明，山顶（Delta）菌株不能产生ST，而OE-PaaflR显示出ST过量生产。这项研究有助于更好地了解ST在P. Anserina，特别是关于其参与真菌生理学。

著录项

  • 来源
    《Environmental microbiology》 |2019年第8期|共16页
  • 作者

    Shen Ling; Poree Francois-Hugues; Gaslonde Thomas; Lalucque Herve; Chapeland-Leclerc Florence; Ruprich-Robert Gwenael;

  • 作者单位

    Univ Paris LIED Univ Paris Diderot UMR 8236 F-75205 Paris France;

    Univ Paris Univ Paris Descartes Lab Pharmacognosie Fac Pharm Paris UMR CNRS CITCOM 8038 F-75006 Paris France;

    Univ Paris Univ Paris Descartes Lab Pharmacognosie Fac Pharm Paris UMR CNRS CITCOM 8038 F-75006 Paris France;

    Univ Paris LIED Univ Paris Diderot UMR 8236 F-75205 Paris France;

    Univ Paris LIED Univ Paris Descartes UMR 8236 F-75205 Paris France;

    Univ Paris LIED Univ Paris Descartes UMR 8236 F-75205 Paris France;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

相似文献

  • 外文文献
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号