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首页> 外文期刊>Environmental microbiology >Influence of form IA RubisCO and environmental dissolved inorganic carbon on the delta C-13 of the clam-chemoautotroph symbiosis Solemya velum
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Influence of form IA RubisCO and environmental dissolved inorganic carbon on the delta C-13 of the clam-chemoautotroph symbiosis Solemya velum

机译：IA Rubisco和环境溶解无机碳形式的影响对蛤化学 - 化学茶甾粒细胞分解洛米松综合体综合体

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Many nutritive symbioses between chemoautotrophic bacteria and invertebrates, such as Solemya velum, have delta(13)C values of similar to-30 to - 35%, considerably more depleted than phytoplankton. Most of the chemoautotrophic symbionts fix carbon with a form IA ribulose 1,5-bisphosphate carboxylase (RubisCO). We hypothesized that this form of RubisCO discriminates against (CO2)-C-13 to a greater extent than other forms. Solemya velum symbiont RubisCO was cloned and expressed in Escherichia coli, purified and characterized. Enzyme from this recombinant system fixed carbon most rapidly at pH 7.5 and 20-25degreesC. Surprisingly, this RubisCO had an epsilon-value (proportional to the degree to which the enzyme discriminates against (CO2)-C-13) of 24.4%, similar to form IB RubisCOs, and higher than form II RubisCOs. Samples of interstitial water from S. velum's habitat were collected to determine whether the dissolved inorganic carbon (DIC) could contribute to the negative delta(13)C values. Solemya velum habitat DIC was present at high concentrations (up to similar to5 mM) and isotopically depleted, with delta(13)C values as low as similar to-6%. Thus environmental DIC, coupled with a high degree of isotopic fractionation by symbiont RubisCO likely contribute to the isotopically depleted delta(13)C values of S. velum biomass, highlighting the necessity of considering factors at all levels (from environmental to enzymatic) in interpreting stable isotope ratios.
机译：诸如Solemala Velum等含有Δ（13）C值的营养营养细菌和无脊椎动物之间的许多营养素有什么营养素与-30至-5％相似，比浮游植物相当更耗尽。大多数化学营养性Symbionts将碳与形式Ia核糖糖丝1,5-双磷酸羧化酶（Rubisco）固定。我们假设这种形式的Rubisco在比其他形式更大程度地歧视（CO2）-C-13。克隆了SoloMYA Velum Symbiont Rubisco并在大肠杆菌中克隆并表达，纯化并表达。来自该重组系统的酶固定在pH 7.5和20-25degreesc上最快的碳。令人惊讶的是，这种Rubisco具有ε-值（与酶鉴定（CO2）-C-13的程度成比例，类似于形式IB Rubiscos，高于II表II rubiscos。收集来自S.Velum的栖息地的间质水的样品，以确定溶解的无机碳（DIC）是否可以有助于负Δ（13）C值。 SoLemya Velum Heabitat DIC以高浓度（最高为5 mm）存在，并且同位素耗尽，DELTA（13）C值与-6％相似。因此，通过Symbiont Rubisco与高度同位素分馏偶联的环境DIC可能有助于S.Velum生物量的同位素耗尽的δ（13）C值，突出了考虑各级因素（从环境到酶）解释的必要性同位素比率稳定。

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