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首页> 外文期刊>European journal of nutrition >Genomic reshuffling in advanced lines of hexaploid tritordeum
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Genomic reshuffling in advanced lines of hexaploid tritordeum

机译：六倍体三倍伟程中先进水线的基因组重新制作

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Genomic restructuring was detected in newly synthesized tritordeum by molecular and cytogenetic tools. Genomic stability is expected for advanced tritordeum lines (H(ch)H(ch)AABB; 2n = 42) with multiple generations of self-fertilization. This study intends to confirm or decline this hypothesis by characterizing three advanced tritordeum lines and their parental species using cytogenetics, inter-simple sequence repeat (ISSR) and retrotransposon-based markers. Mitotic chromosomes of each tritordeum line were hybridized with six synthetic oligonucleotide probes using non-denaturing fluorescence in situ hybridization. Polymorphic hybridization patterns and structural rearrangements involving SSR regions were detected. The same chromosome spreads were re-hybridized with genomic DNA of Hordeum chilense Roem. et Schult. and the 45S ribosomal DNA (rDNA) sequence pTa71. These FISH experiments allowed for parental genome discrimination, identification of nucleolar chromosomes, and detection of structural rearrangements, mostly involving rDNA loci. The chromosomes bearing SSR hybridization signals and/or chromosomes involved in structural rearrangements were identified. ISSR, retrotransposon-microsatellite amplified polymorphism, inter-retrotransposon amplified polymorphism and inter-primer binding site markers evidenced genomic reshuffling in all tritordeum lines relative to their parents. Line HT28 was considered the most genetically stable. This work demonstrated that cytogenetic and molecular monitoring of tritordeum is needed, even after several self-fertilization generations, to guarantee the selection of the most stable lines for improvement and sustainable agriculture.
机译：通过分子和细胞遗传学工具在新合成的三角组中检测到基因组重组。预期基因组稳定性预期高级三萜系（H（CH）H（CH）AABB; 2N = 42），具有多一代自施。本研究打算通过使用细胞遗传学，简单简单的序列重复（ISSR）和基于RORTORANSPOSON的标记来表征三条先进的三萜系和他们的家庭来确认或拒绝这一假设。使用非变性荧光原位杂交用六种合成的寡核苷酸探针杂交，每个三角型线的丝状染色体杂交。检测涉及SSR区域的多态性杂交模式和结构重排。与Hordeum Chilense Roem的基因组DNA重新杂交相同的染色体差异。埃尔舒勒。和45s核糖体DNA（RDNA）序列PTA71。这些鱼类实验允许父母基因组鉴别，核磁染色体的鉴定，以及检测结构重排，主要涉及RDNA基因座。鉴定了轴承SSR杂交信号和/或涉及结构重排的染色体。 ISSR，回析烷烃微卫星扩增多态性，再横向横向蛋白酶扩增的多态性和引物间结合位点标记证明了相对于父母的所有三角型线条中的基因组重新洗脱。线HT28被认为是最遗传稳定的。这项工作表明，即使经过几代自施剂，也需要三核的细胞遗传学和分子监测，以确保选择最稳定的改善和可持续农业的线条。

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