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首页> 外文期刊>Immunology: An Official Journal of the British Society for Immunology >CD73 expression identifies a subset of IgM(+) antigen-experienced cells with memory attributes that is T cell and CD40 signalling dependent
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CD73 expression identifies a subset of IgM(+) antigen-experienced cells with memory attributes that is T cell and CD40 signalling dependent

机译：CD73表达式识别具有内存属性的IGM（+）抗原经验丰富的单元的子集，即是T细胞和CD40信令相关的

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B-cell memory was long characterized as isotype-switched, somatically mutated and germinal centre (GC)-derived. However, it is now clear that the memory pool is a complex mixture that includes unswitched and unmutated cells. Further, expression of CD73, CD80 and CD273 has allowed the categorization of B-cell memory into multiple subsets, with combinatorial expression of the markers increasing with GC progression, isotype-switching and acquisition of somatic mutations. We have extended these findings to determine whether these markers can be used to identify IgM memory phenotypically as arising from T-dependent versus T-independent responses. We report that CD73 expression identifies a subset of antigen-experienced IgM(+) cells that share attributes of functional B-cell memory. This subset is reduced in the spleens of T-cell-deficient and CD40-deficient mice and in mixed marrow chimeras made with mutant and wild-type marrow, the proportion of CD73(+) IgM memory is restored in the T-cell-deficient donor compartment but not in the CD40-deficient donor compartment, indicating that CD40 ligation is involved in its generation. We also report that CD40 signalling supports optimal expression of CD73 on splenic T cells and age-associated B cells (ABCs), but not on other immune cells such as neutrophils, marginal zone B cells, peritoneal cavity B-1 B cells and regulatory T and B cells. Our data indicate that in addition to promoting GC-associated memory generation during B-cell differentiation, CD40-signalling can influence the composition of the unswitched memory B-cell pool. They also raise the possibility that a fraction of ABCs may represent T-cell-dependent IgM memory.
机译：B细胞内存长，表征为同学切换，有组有细胞突变和生发中心（GC）。但是，现在可以清楚地清楚的是，内存池是一种复杂的混合物，包括未开关和未传养的细胞。此外，CD73，CD80和CD273的表达允许将B细胞存储器分类为多个亚群，具有COMINATIAL表达与GC进展，同种型切换和躯体突变的获取增加。我们已经扩展了这些发现，以确定这些标记是否可用于鉴定IGM记忆表型，因为从T依赖于T无关的反应而产生。我们报告了CD73表达式标识了共享功能性B单元存储器的属性的抗原经验的IGM（+）单元的子集。该子集在T细胞缺陷和CD40缺陷小鼠的脾脏中减少，并且在用突变体和野生型骨髓制造的混合骨髓嵌合体中，CD73（+）IgM记忆的比例在T细胞缺陷中恢复捐赠室但不在CD40缺陷的供体室中，表明CD40连接参与其一代。我们还报告了CD40信号传导支持CD73对脾脏T细胞和年龄相关的B细胞（ABC）的最佳表达，但不是在其他免疫细胞上，例如中性粒细胞，边缘区B细胞，腹膜腔B-1b细胞和调节器和b细胞。我们的数据表明，除了在B细胞分化期间促进GC相关的存储器产生之外，CD40信令可以影响未开关的存储器B细胞库的组成。它们还提出了ABC的一部分可以代表T细胞依赖性IGM存储器的可能性。

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