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首页> 外文期刊>European Journal of Plant Pathology >Effects of green light on the gene expression and virulence of the plant pathogen Pseudomonas cichorii JBC1
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Effects of green light on the gene expression and virulence of the plant pathogen Pseudomonas cichorii JBC1

机译：绿光对植物病原体甲米西氏菌的基因表达及毒力的影响

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摘要

Increasing evidence suggests that the interactions between plant and pathogen are influenced by light perception of both organisms. In our previous study, green light decreased disease severity in tomato plants in response to Pseudomonas cichoriiJBC1 (PcJBC1) infection via induction of disease resistance-related genes. However, the influence of green light on gene expression and metabolism of PcJBC1 has not been explored. In this study, we cultured PcJBC1 in hrp-inducing minimal media (MM) under green light and dark condition, and analyzed the expression of genes, which are important in pathogenicity/virulence and epiphytic survival by real-time PCR (qPCR). Although no significant changes were observed in the expression of type 3 secretion system (T3SS)- and flagellar-related genes in response to green light, genes for the production of phytotoxic lipopeptides and siderophores were significantly reduced by green light. In addition, the phytotoxic lipopeptide and siderophore production in the culture supernatant was consistent with the results of gene expression. Furthermore, we compared the whole transcriptomes of PcJBC1 grown in MM under green light and dark condition. Among 243 differentially expressed genes (& 2-fold change), the photoreceptor genes, bacteriophytochrome (bphP) and heme-oxygenase (bphO) were significantly up-regulated, whereas genes involved in the type 1 secretion system (T1SS), type 6 secretion system (T6SS), phytotoxic lipopeptides, and iron acquisition were profoundly repressed under green light. Corresponding to qPCR analysis, the RNA-seq results also showed no significant alteration in the T3SS- and flagellar-related genes. Overall, our results suggest that green light perceived by PcJBC1 plays a key role in diverse physiological responses that might affect this pathogen's epiphytic survival. The receptor and signaling network involved in green light perception should be identified in further investigation.
机译：越来越多的证据表明，植物和病原体之间的相互作用受到对两种生物的轻微感知的影响。在我们以前的研究中，绿灯响应于抗病抗性相关基因的假单胞菌CICHORIIJBC1（PCJBC1）感染而降低了番茄植物的疾病严重程度。然而，尚未探讨绿光对PCJBC1基因表达和代谢的影响。在这项研究中，我们在绿光和暗状态下培养了PCJBC1在HRP诱导的最小培养基（MM）中，并分析了基因的表达，这是通过实时PCR（QPCR）的致病性/毒力和副磷脂等重要性。尽管在表达3型分泌系统（T3SS）的表达中没有显着变化，但是响应于绿光的鞭毛相关基因，但通过绿光显着降低了生产植物脂肽脂肽和施工会的基因。此外，培养上清液中的植物毒性脂肽和纵向产生与基因表达的结果一致。此外，我们将PCJBC1的整个转录组与绿光和暗条件下的MM生长的整个转录组进行了比较。在243个差异表达的基因（＆amp; 2倍）中，感光体基因，噬菌体（BphP）和血红素 - 氧酶（Bpho）显着上调，而第1型分泌系统（T1S）的基因，在绿光下，型式6分泌系统（T6SS），植物毒性脂肽和铁采集是深受抑制的。对应于QPCR分析，RNA-SEQ结果也显示出T3S-和鞭毛相关基因的显着改变。总体而言，我们的结果表明，PCJBC1所感知的绿灯在不同的生理反应中起关键作用，可能影响该病原体的果实生存率。应在进一步调查中识别涉及绿光感知的受体和信号网络。

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