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首页> 外文期刊>European Journal of Plant Pathology >Bimetallic blends and chitosan nanocomposites: novel antifungal agents against cotton seedling damping-off
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Bimetallic blends and chitosan nanocomposites: novel antifungal agents against cotton seedling damping-off

机译：双金属混合物和壳聚糖纳米复合材料：对棉花幼苗抑制的新型抗真菌剂

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Phytopathological studies of chitosan nanocomposites are mainly focused on in vitro efficiency, so it is essential to perform a complementary greenhouse assay to find eco-friendly alternatives for plant disease management. In the present study, Cu-chitosan and Zn-chitosan nanocomposites were prepared by reduction of metal precursors in the presence of chitosan in sc CO2 medium and deposition of organosol on chitosan, respectively. Physicochemical properties of the nanocomposites were characterized by X-ray fluorescence analysis (XRF), Small angles X-ray Scattering (SAXS), X-ray Photoelectron spectroscopy (XPS), and Transmission electron microscopy (TEM). The bimetallic blends (BBs) based on nanoscale Cu(OH)(2) were obtained through simple precipitation and grinding methods. In vitro and in vivo studies of the antifungal activity of Cu-chitosan, Zn-chitosan and BBs at concentrations of 30, 60, and 100 mu g ml(-1) were conducted against two anastomosis groups of Rhizoctonia solani for control of cotton seedling damping-off. Effect of metal-chitosan nanocomposites at 100 mu g ml(-1) combined with Cu-tolerant Trichoderma longibrachiatum strains was also evaluated for control of cotton seedling damping-off under greenhouse conditions. The BBs and Cu-chitosan nanocomposite showed the highest antifungal efficacy against both anastomosis groups of R. solani in vitro. These results indicated that BBs, Cu-chitosan nanocomposite, and BBs combined with Trichoderma may suppress cotton seedling disease caused by R. solani in vivo. The evaluation of R. solani in a greenhouse with a Trichoderma strain showed synergistic inhibitory effect with BBs. Light micrographs of mycelia treated with BBs showed the disruption of the hyphal structures. The interaction of the nanocomposites with DNA isolated from the exposed fungal cells, by means of bonding and/or degradation, was also investigated. DNA interaction in terms of binding and degradation for treated DNA with BBs and chitosan nanocomposites was demonstrated. The results showed the absence of DNA amplification by a microsatellite primed PCR.
机译：壳聚糖纳米复合材料的植物病理学研究主要集中于体外效率，因此必须进行互补的温室测定，以寻找生态友好的植物疾病管理替代品。在本研究中，通过在SC CO 2培养基中的壳聚糖存在下还原金属前体并分别在壳聚糖上沉积有机溶胶，通过减少金属前体来制备Cu-Chotosan和Zn-壳聚糖纳米复合材料。纳米复合材料的物理化学性质的特征在于X射线荧光分析（XRF），小角X射线散射（SAX），X射线光电子能谱（XPS）和透射电子显微镜（TEM）。通过简单的沉淀和研磨方法获得基于纳米级Cu（OH）（2）的双金属共混物（BBS）。在体外和体内研究Cu-壳聚糖的抗真菌活性，Zn-壳聚糖和BBS的浓度为30,60和100μgmm（-1），用于对棉幼苗进行控制的两个吻合组阻尼。还评估了金属 - 壳聚糖纳米复合材料在100μgml（-1）中与Cu耐受性的Trichoderma长刺菌株的影响进行了评价温室条件下棉花幼苗阻尼的控制。 BBS和Cu-Chitosan纳米复合物在体外对R.Solani的吻合组群抗真菌药效果最高。这些结果表明，BBS，Cu-Chotosan纳米复合材料和BBS与Trichoderma联合的BBS可以抑制由体内r.Solani引起的棉花幼苗疾病。具有Trichoderma菌株的温室中R. Solani的评价显示出与BBS的协同抑制作用。用BBS治疗的菌丝菌丝菌丝菌丝显示出亚腿结构的破坏。还研究了纳米复合材料与从暴露的真菌细胞中分离的DNA的相互作用，通过键合和/或降解，通过键合和/或降解。证实了用BBS和壳聚糖纳米复合材料对处理DNA的结合和降解的DNA相互作用。结果表明，微卫星引发的PCR没有DNA扩增。

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