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首页> 外文期刊>European Journal of Plant Pathology >Fast detection by loop-mediated isothermal amplification (LAMP) of the three begomovirus species infecting tomato in Panama
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Fast detection by loop-mediated isothermal amplification (LAMP) of the three begomovirus species infecting tomato in Panama

机译：通过在巴拿马中排序番茄三种Emmomovirus种类的环介导的等温扩增（灯）的快速检测

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摘要

Potato yellow mosaic Panama virus (PYMPV), Tomato leaf curl Sinaloa virus (ToLCSiV) and Tomato yellow mottle virus (TYMoV) of genus Begomovirus (family Geminiviridae) are the only three begomovirus species detected infecting tomato (Solanum lycopersicum L.) in Panama. PYMPV, ToLCSiV and TYMoV induce symptoms of stunting, yellowing, curling, distortion of leaves and reduction of fruit size and cause important economic loses. A loop-mediated amplification under isothermal conditions (LAMP) assay was developed for the individual detection of these three begomovirus species by using a set of three primer pairs specific per each one of them. Amplification products were visualized by gel electrophoresis or direct Gel-Red staining of DNA into the reaction tube. PYMPV, ToLCSiV and TYMoV were detected in total DNA extracts obtained from different plant tissues such as leaves, stems, flowers, fruits and roots of infected tomato plants collected in different production regions of Panama. LAMP sensitivity was similar to that of conventional PCR but, the first procedure was faster and cheaper than the last one. Moreover, all three viruses were successfully detected by LAMP and not by conventional PCR from sap extracts obtained from leaf tissues of infected tomato plants which were embedded into 3MM Whatman paper and stored several days, facilitating the samples processing as well as the material movement among different laboratories. Therefore, LAMP is a specific, rapid and cheap procedure to detect all three begomoviruses infecting tomato in Panama and it is suitable for field surveys and sanitation programs.
机译：马铃薯黄色马赛克巴拿马病毒（Pympv），番茄叶卷曲SILLOA病毒（TOTCSIV）和Begomovirus（Geminiviridae家族）的番茄黄色斑驳病毒（Tymov）是在巴拿马中检测到番茄（Solanum Lycopersicum L.）的唯一检测到的草原动物物种。 PMPV，Tolcsiv和Tymov诱导令人生畏的症状，炎热，卷曲，叶片的畸变和果实的减少，并导致经济的重要失败。通过使用每个每一个特异性的三种引物对，开发出在等温条件（灯）测定下的环介导的扩增用于单独检测这三个原毛病毒物种。通过凝胶电泳或直接凝胶 - 红色染色DNA将扩增产物视为反应管。在不同植物组织中获得的总DNA提取物中检测到PMPV，Tolcsiv和Tymov，例如在巴拿马的不同生产区域收集的感染番茄植物的叶子，茎，花，花，水果和植物，如叶，茎，花，水果和根部。灯敏感性与传统PCR相似，但是，第一个程序比上一个程序更快和便宜。此外，所有三种病毒通过灯成功检测，而不是由常规的PCR从被感染的番茄植物的叶组织中获得的SAP提取物，该番茄植物嵌入3mm whinman纸上并储存几天，便于样品处理以及不同的材料运动实验室。因此，灯是一种特定的，快速且廉价的程序，用于检测在巴拿马感染番茄的所有三个原毛病，它适用于现场调查和卫生计划。

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