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首页> 外文期刊>European Journal of Plant Pathology >Genotypic and phenotypic variability of Pectobacterium strains causing blackleg and soft rot on potato in Turkey
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Genotypic and phenotypic variability of Pectobacterium strains causing blackleg and soft rot on potato in Turkey

机译：土耳其马铃薯诱导黑杆菌菌株的基因型和表型变异性

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摘要

Pectinolytic bacteria were isolated from 48 potato plants showing the symptoms of blackleg and collected in different fields of commercial potato production areas at Samsun, Amasya, Corum and Yozgat provinces in Turkey in 2015. The survey resulted in the isolation of 26 pectinolytic strains that belonged to P. atrosepticum, P. carotovorum subsp. brasiliense, P. carotovorum subsp. carotovorum and P. parmentieri species based on molecular identification with species-specific PCR and phenotypic characterization. The identified strains indicated typical biochemical characteristics of the assigned species. For 16 representative Pectobacterium isolates 10 unique rep-PCR band patterns were obtained. The 16S rRNA and recA and gapA gene fragment sequencing confirmed the species identity of the isolates. The phenotypic characterization of the strains revealed that for all assays but one (cellulase, protease activity, swimming but not swarming), the tested Pectobacterium species were significantly different from each other proving the diversity of the strains belonging to these genera. Recent outbreaks of blackleg and/or soft rot in potato production areas in Turkey may pose a threat on other crops, as tomato, pepper, cucumber, onion, cabbage, broccoli and sugar beet are cultivated in the same provinces.
机译：从48种马铃薯植物中分离出果解细菌，显示Blackleg的症状，并于2015年在土耳其的萨姆松，阿马西亚，哥斯兰和Yozgat省份的商业马铃薯生产区域的不同领域收集。该调查导致分离属于26种果胶溶液P.Atrosepticum，P.Carotovorum亚普。 Brasiliense，P.Carotovorum Subsp。基于物种特异性PCR和表型表征的基于分子鉴定的Carotovorum和P.Parmentieri物种。所识别的菌株表明了分配的物种的典型生化特征。对于16个代表性的胶质杆菌分离物，获得了10个独特的REP-PCR带状图案。 16S RRNA和RECA和GAPA基因片段测序证实了分离株的物种标识。菌株的表型表征显示，对于所有测定但是一种（纤维素酶，蛋白酶活性，游泳但不蜂拥而至），测试的胶杆物种彼此显着不同，证明了属于这些属的菌株的多样性。最近爆​​发土耳其土豆生产区的Blackleg和/或软腐烂可能会对其他作物构成威胁，因为番茄，胡椒，黄瓜，洋葱，白菜，西兰花和甜菜在同一个省份中培养。

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