...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>European Journal of Plant Pathology >Evaluation of PCR markers for Phytophthora infestans mating type determination
【24h】

Evaluation of PCR markers for Phytophthora infestans mating type determination

机译：对PCR培养物患有交配类型测定的PCR标志物的评价

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Late blight is a devastating potato disease caused by Phytophthora infestans. This organism can reproduce asexually and sexually between the strains of two mating types named A1 and A2. Mating type is an important strain characteristic affecting the pathogen's population structure. We validate different PCR markers for P. infestans mating type determination by comparison of the results obtained with the markers (W16, S1, PHYB) with the pairing test results for 26 isolates collected worldwide and for a group of 146 Polish isolates. This study identifies an interesting feature of the isolates of genotype US-1. For all these A1 mating type isolates, the product specific for A2 isolates is amplified using the marker W16. Analysis of sequences of W16 PCR products indicates high similarity of the US-1 isolates with modern A2 mating type isolates. When US-1 isolates are excluded from analysis, 95 and 96% of isolates are correctly assigned by markers W16 and S1, respectively, when compared with the pairing test results. Marker PHYB produces 14% of discrepant results with the pairing test. Our results show that molecular markers can be useful tools for P. infestans mating type determination, but their application should be preceded by validation in each local population since their efficiency may vary depending on a pathogen's genotype.
机译：晚期枯萎是一种由植物炎患者引起的毁灭性的马铃薯疾病。这种有机体可以在名为A1和A2的两个配合类型的菌株之间进行无性繁殖和性行为。交配类型是影响病原体人口结构的重要菌株特征。我们通过将标记物（W16，S1，PHYB）与全球收集的26个分离物的配对测试结果进行比较，验证P. infestans交配类型测定的不同PCR标志物。该研究识别出基因型US-1分离株的有趣特征。对于所有这些A1配合型分离株，使用标记W16放大特异于A2分离株的产品。 W16 PCR产物序列分析表明US-1与现代A2配合型分离株的高相似性。当US-1分离株被排除在分析之外，与配对测试结果相比，分别通过标记W16和S1正确分配95和96％的分离物。标记PHYB通过配对测试产生14％的差异结果。我们的结果表明，分子标记可以是P.Infestans交配类型测定的有用工具，但它们的应用在每个局部群体中应在验证之前，因为它们的效率可能因病原体的基因型而异。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号