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首页> 外文期刊>Advances in Experimental Medicine and Biology >Human lactoferrin in the milk of transgenic mice increases intestinal growth in ten-day-old suckling neonates.
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Human lactoferrin in the milk of transgenic mice increases intestinal growth in ten-day-old suckling neonates.

机译：转基因小鼠乳汁中的人乳铁蛋白可增加十日龄哺乳期新生儿的肠道生长。

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Regulatory roles and a signaling receptor have been proposed for the milk protein lactoferrin (Lf), but none has been definitively characterized. Nichols and colleagues (1987) observed that human lactoferrin (hLf) stimulated thymidine incorporation into the DNA of rat intestinal crypt cells. We tested the hypothesis that chronic Lf administration stimulates intestinal growth by studying neonatal mice suckling transgenic dams secreting about 12 mg/mL hLf in their milk. Specifically, nontransgenic litters were adjusted to eight pups each and cross-fostered to transgenic dams. Controls were pups suckling nontransgenic dams of the same strain. On day 10 postpartum pups were weighed, sacrificed, and the small intestines were weighed, measured, and stored for later determination of enzyme activities. The results indicate that intestinal growth was increased in neonates suckling transgenic dams. The weight of the small intestine was increased about 27% when the pups received milk containing hLf. Intestinal length only increased about 6.5% suggesting that Lf in milk enhanced mucosal growth. The ratio of maltase to lactase in the duodenal segment of the small intestine, an indicator of maturation, was also significantly increased in the pups suckling transgenic milks. Our results imply that chronic oral consumption of human Lf promotes the growth and maturation of the intestinal mucosa, and suggest a possible therapeutic role for the agent in premature infants as well as in patients with bowel damage.
机译：已经提出了乳蛋白乳铁蛋白（Lf）的调节作用和信号受体，但尚未明确鉴定。 Nichols和同事（1987）观察到人乳铁蛋白（hLf）刺激胸腺嘧啶核苷掺入大鼠肠隐窝细胞的DNA中。我们通过研究新生小鼠哺乳在乳汁中分泌约12 mg / mL hLf的转基因大坝的新生小鼠，验证了长期Lf施用刺激肠道生长的假说。具体而言，将非转基因垫料每个调整为八只幼崽，并交叉育成转基因大坝。对照是哺乳同一菌株的非转基因大坝的幼崽。在第10天，称重，处死幼崽，称重，测量并储存小肠，以用于以后的酶活性测定。结果表明，在哺乳转基因大坝的新生儿中肠的生长增加了。当幼犬接受含hLf的牛奶时，小肠的重量增加了约27％。肠长仅增加约6.5％，表明牛奶中的Lf增强了粘膜生长。在哺乳转基因乳的幼崽中，小肠十二指肠段中麦芽糖酶与乳糖酶的比率（成熟的指标）也显着增加。我们的结果表明，长期口服人类Lf会促进肠道粘膜的生长和成熟，并提示该药物在早产儿和肠损伤患者中可能具有治疗作用。

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