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首页> 外文期刊>European radiology >Gd-EOB-DTPA-enhanced MRI for quantitative assessment of liver organ damage after partial hepatic ischaemia reperfusion injury: correlation with histology and serum biomarkers of liver cell injury
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Gd-EOB-DTPA-enhanced MRI for quantitative assessment of liver organ damage after partial hepatic ischaemia reperfusion injury: correlation with histology and serum biomarkers of liver cell injury

机译：GD-Eob-DTPA增强MRI用于肝脏缺血再灌注损伤后肝脏器官损伤的定量评估：与肝细胞损伤的组织学和血清生物标志物的相关性

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摘要

ObjectiveTo evaluate Gd-EOB-DTPA-enhanced MRI for quantitative assessment of liver organ damage after hepatic ischaemia reperfusion injury (IRI) in mice.MethodsPartial hepatic IRI was induced in C57Bl/6 mice (n = 31) for 35, 45, 60 and 90 min. Gd-EOB-DTPA-enhanced MRI was performed 1 day after surgery using a 3D-FLASH sequence. A subgroup of n = 9 animals with 60 min IRI underwent follow-up with MRI and histology 7 days after IRI. The total liver volume was determined by manual segmentation of the entire liver. The volume of functional, contrast-enhanced liver parenchyma was quantified by a region growing algorithm (visual threshold) and an automated segmentation (Otsu's method). The percentages of functional, contrast-enhanced and damaged non-enhanced parenchyma were calculated according to these volumes. MRI data was correlated with serum liver enzyme concentrations and histologically quantified organ damage using periodic acid-Schiff (PAS) staining.ResultsThe percentage of functional (contrasted) liver parenchyma decreased significantly with increasing ischaemia times (control, 94.4 3.3%; 35 min IRI, 89.3 4.1%; 45 min IRI, 87.9 3.3%; 60 min IRI, 68 10.5%, p 0.001 vs. control; 90 min IRI, 55.9 11.5%, p 0.001 vs. control). The percentage of non-contrasted liver parenchyma correlated with histologically quantified liver organ damage (r = 0.637, p 0.01) and serum liver enzyme elevations (AST r = 0.577, p 0.01; ALT r = 0.536, p 0.05). Follow-up MRI visualized recovery of functional liver parenchyma (71.5 +/- 8.7% vs. 84 +/- 2.1%, p 0.05), consistent with less histological organ damage on day 7.ConclusionWe demonstrated the feasibility of Gd-EOB-DTPA-enhanced MRI for non-invasive quantification of damaged liver parenchyma following IRI in mice. This novel methodology may refine the characterization of liver disease and could have application in future studies targeting liver organ damage.
机译：ObjectiveTo评估GD-Eob-DTPA - 增强MICE治疗肝脏再灌注损伤（IRI）后的肝脏器官损伤的定量评估MIRI。方法在C57BL / 6小鼠（N = 31）中诱导35,45,60和90分钟。使用3D闪存序列，手术后1天进行GD-Eob-DTPA增强MRI。 N = 9只动物的亚组，60分钟内脏的IRI在IRI后7天进行MRI和组织学后续随访。通过整个肝脏的手动分割来确定总肝脏体积。通过区域生长算法（视觉阈值）和自动分割（OTSU的方法）量化功能性，对比度增强肝实质体积。根据这些体积计算功能性，对比度增强和损坏的非增强薄壁型的百分比。 MRI数据与血清肝酶浓度和使用周期性酸 - 席夫（PAS）染色的组织学定量器官损伤相关。函数（对比）肝实质的百分比随着缺血时间的增加而显着降低（对照，94.4 3.3％; 35分钟， 89.3 4.1％; 45分钟IRI，87.9 3.3％; 60分钟IRI，68.5％，P <0.001对照; 90分钟IRI，55.91.5％，P <0.001对照）。与组织学定量肝脏器官损伤相关的非对比性肝实质的百分比（r = 0.637，p <0.01）和血清肝酶凸起（AST r = 0.577，P <0.01; ALT r = 0.536，P <0.05 ）。随访MRI可视化函数肝实质的恢复（71.5 +/- 8.7％与84 +/- 2.1％，P <0.05），与第7天的组织学器官损伤较少.ConclusionWe呈现GD-EOB的可行性-DTPA增强MRI用于小鼠IRI后IRI后的受损肝实质的非侵入性定量。这种新的方法可以优化肝病的表征，并且可以在未来的研究中具有针对肝脏器官损伤的研究。

著录项

  • 来源
    《European radiology》 |2018年第10期|共10页
  • 作者

    Getzin Tobias; Gueler Faikah; Hartleben Bjoern; Gutberlet Marcel; Thorenz Anja; Chen Rongjun; Meier Martin; Braesen Jan Hinrich; Derlin Thorsten; Hartung Dagmar; Lang Hannah A. S.; Haller Hermann; Wacker Frank; Rong Song; Hueper Katja;

  • 作者单位

    Hannover Med Sch Diagnost &

    Intervent Radiol Carl Neuberg Str 1 D-30625 Hannover Germany;

    Hannover Med Sch Nephrol Carl Neuberg Str 1 D-30625 Hannover Germany;

    Hannover Med Sch Pathol Carl Neuberg Str 1 D-30625 Hannover Germany;

    Hannover Med Sch Diagnost &

    Intervent Radiol Carl Neuberg Str 1 D-30625 Hannover Germany;

    Hannover Med Sch Nephrol Carl Neuberg Str 1 D-30625 Hannover Germany;

    Hannover Med Sch Nephrol Carl Neuberg Str 1 D-30625 Hannover Germany;

    Hannover Med Sch Inst Lab Anim Sci Carl Neuberg Str 1 D-30625 Hannover Germany;

    Hannover Med Sch Pathol Carl Neuberg Str 1 D-30625 Hannover Germany;

    Hannover Med Sch Nucl Med Carl Neuberg Str 1 D-30625 Hannover Germany;

    Hannover Med Sch Diagnost &

    Intervent Radiol Carl Neuberg Str 1 D-30625 Hannover Germany;

    Hannover Med Sch Diagnost &

    Intervent Radiol Carl Neuberg Str 1 D-30625 Hannover Germany;

    Hannover Med Sch Nephrol Carl Neuberg Str 1 D-30625 Hannover Germany;

    Hannover Med Sch Diagnost &

    Intervent Radiol Carl Neuberg Str 1 D-30625 Hannover Germany;

    Hannover Med Sch Nephrol Carl Neuberg Str 1 D-30625 Hannover Germany;

    Hannover Med Sch Diagnost &

    Intervent Radiol Carl Neuberg Str 1 D-30625 Hannover Germany;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 放射医学;
  • 关键词

    Gadolinium ethoxybenzyl DTPA; Magnetic resonance imaging; Liver; Ischaemia reperfusion injury; Mice;

    机译：钆乙氧基苄基DTPA;磁共振成像;肝脏;缺血再灌注损伤;小鼠;

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