...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Experimental and therapeutic medicine >Effects of the surface characteristics of nanoporous titanium oxide films on Ti-24Nb-4Zr-8Sn alloy on the initial adhesion of osteoblast-like MG-63 cells
【24h】

Effects of the surface characteristics of nanoporous titanium oxide films on Ti-24Nb-4Zr-8Sn alloy on the initial adhesion of osteoblast-like MG-63 cells

机译：纳米多氧化钛膜对Ti-24NB-4ZR-8SN合金对成骨细胞样MG-63细胞初始粘合性的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The aim of the present study was to investigate the effects of the surface characteristics of nanoporous titanium oxide films, formed by anodization on Ti-24Nb-4Zr-8Sn (Ti2448) alloy, on the early adhesion of osteoblast-like MG-63 cells. Nanoporous titanium oxide films with two different pore sizes (30 and 90 nm) were formed by anodization in NH4F solution on Ti2448 alloy. The surface roughness of the nanoporous titanium oxide films was determined using a Surftest Formtracer and field emission scanning electron microscopy (FESEM). Cell viability was evaluated at different time points using the 3-(4,5-dimethylthiazol-2-y1)-2,5-diphenyltetrazolium bromide (MTT) assay. To investigate the regulatory mechanisms involved in the focal adhesion of osteoblasts to Ti2448 alloy, we quantified the expression levels of integrin beta 1 and paxillin mRNAs on the nanoporous titanium oxide films during early osteoblast adhesion using real-time RT-PCR. Samples with a 30-nm nanoporous film exhibited a greater number of overlapping microporous structures with microprojections compared with the 90-nm nanoporous film samples. The MTT assay indicated that cell viability on the 30-nm nanoporous surface following 24 and 48 h of cell culture was higher than those observed on the unanodized control and 90-nm nanoporous surfaces. Integrin beta 1 mRNA expression levels on the 30-nm nanoporous surface following cell culture for 48 h were also significantly higher compared with those on the unanodized control and 90-nm nanoporous surfaces. The results demonstrated that a 30-nm nanoporous titanium oxide filth on Ti2448 alloy may provide the optimum bioactive implant surface for the. initial adhesion of osteoblasts.
机译：本研究的目的是研究纳米多氧化钛氧化膜的表面特性的影响，在Ti-24NB-4ZR-8SN（Ti2448）合金上通过阳极氧化，在成骨细胞样MG-63细胞的早期粘附性上。通过在Ti2448合金的NH4F溶液中阳极氧化形成具有两种不同孔径（30和90nm）的纳米孔钛氧化膜。使用表面排放扫描电子显微镜（FESEM）测定纳米多孔氧化钛膜的表面粗糙度。使用3-（4,5-二甲基噻唑-2- y1）-2,5-二苯基四唑溴（MTT）测定在不同的时间点在不同的时间点评价细胞活力。为了探讨参与成骨细胞的局灶性粘附到Ti2448合金的调节机制，我们使用实时RT-PCR在早期成骨细胞粘附期间量化纳米多孔钛氧化膜上整合蛋白β1和帕雪林MRNA的表达水平。与30nm纳米多孔膜样品相比，具有30nm纳米多孔膜的样品具有更多数量的具有微型微孔的微孔结构。 MTT测定表明，在细胞培养物的30-nm纳米多孔表面上的细胞活力高于在未约束对照和90nm纳米多孔表面上观察到的。与未混合对照和90nm纳米多孔表面相比，在细胞培养物后，在细胞培养物后，在细胞培养物后，30nm纳米电孔表面的MRNA表达水平也显着高。结果表明，Ti2448合金上的30nm纳米氧化钛氧化物可以为其提供最佳的生物活性植入物。成骨细胞的初始粘附性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号