...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Experimental and therapeutic medicine >Protective role of microRNA-219-5p inhibitor against spinal cord injury via liver receptor homolog-1/Wnt/-catenin signaling pathway regulation
【24h】

Protective role of microRNA-219-5p inhibitor against spinal cord injury via liver receptor homolog-1/Wnt/-catenin signaling pathway regulation

机译：MicroRNA-219-5P抑制剂对脊髓损伤抗脊髓损伤的保护作用通过肝受体同源物 - 1 / Wnt / -catenin信号通路调节

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The present study aimed to investigate the role of microRNA (miR)-219-5p in spinal cord injury (SCI) and to examine the underlying molecular mechanism. SCI rat and cell models were conducted in the current study, while reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the level of miR-219-5p in the SCI mice and neurons. Bioinformatics analysis was applied to predict the target genes of miR-219-5p, and dual-luciferase reporter assay was performed to verify the prediction. In addition, MTT assay and flow cytometry were conducted to determine the cell viability and cell apoptosis of the neurons, respectively. Western blot analysis was also performed to detect the expression of associated proteins. The study results demonstrated that miR-219-5p was highly expressed in SCI mice and neurons, and directly targets liver receptor homolog-1 (LRH-1). The neuron viability was significantly reduced by SCI, however, it was recovered upon transfection with an miR-219-5p inhibitor. Neuron apoptosis was notably induced by SCI and inhibited by miR-219-5p inhibition. The LRH-1/Wnt/-catenin signaling pathway was also inhibited by SCI, while it was significantly enhanced by the miR-219-5p inhibitor. Furthermore, LRH-1 overexpression eliminated the effects of the miR-219-5p inhibitor on SCI. In conclusion, these data indicated that the miR-219-5p inhibitor served a protective role in SCI via regulating the LRH-1/Wnt/-catenin signaling pathway.
机译：本研究旨在探讨MicroRNA（MIR）-219-5P在脊髓损伤（SCI）中的作用，并检查潜在的分子机制。在目前的研究中进行了SCI大鼠和细胞模型，而逆转录量定量聚合酶链反应（RT-QPCR）用于检测SCI小鼠和神经元中miR-219-5p的水平。施用生物信息学分析以预测miR-219-5p的靶基因，并进行双荧光素酶报告结果以验证预测。另外，进行MTT测定和流式细胞术以分别确定神经元的细胞活力和细胞凋亡。还进行了Western印迹分析以检测相关蛋白的表达。研究结果表明，MIR-219-5P在SCI小鼠和神经元中高度表达，直接靶向肝受体同源物 - 1（LRH-1）。通过SCI显着降低神经元活力，但是，在用miR-219-5p抑制剂转染时被回收。神经元细胞凋亡由SCI特别诱导并抑制miR-219-5p抑制。 SCI还抑制了LRH-1 / Wnt / -catenin信号传导途径，而MiR-219-5P抑制剂显着增强。此外，LRH-1过表达消除了MIR-219-5P抑制剂对SCI的影响。总之，这些数据表明MIR-219-5P抑制剂通过调节LRH-1 / WNT / -CatenIn信号传导途径在SCI中提供了保护作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号