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首页> 外文期刊>Experimental and therapeutic medicine >The expression level of miR-155 in plasma and peripheral blood mononuclear cells in coronary artery disease patients and the associations of these levels with the apoptosis rate of peripheral blood mononuclear cells
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The expression level of miR-155 in plasma and peripheral blood mononuclear cells in coronary artery disease patients and the associations of these levels with the apoptosis rate of peripheral blood mononuclear cells

机译：冠状动脉疾病患者血浆和外周血单核细胞miR-155的表达水平及这些水平与外周血单核细胞凋亡率的关联

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摘要

The aim of the study was to investigate the expression of miR-155 in plasma and peripheral blood mononuclear cells (PBMCs), the effects of miR-155 on the apoptosis rate of PBMCs and the extent of coronary stenosis in coronary artery disease patients. Seventy chest pain patients were divided into three groups by symptoms, signs, auxiliary examination and coronary arteriography: 21 cases in the acute myocardial infarction group (AMI), 23 cases in the Angor pectoris group (AP) and 26 cases in the control group (CT). The peripheral blood mononuclear cells of the patients in the three groups were separated for cell culture. Annexin V/propidium iodide (PI) was performed to analyze cell apoptosis. RT-qPCR was used to evaluate the expression level of miR-155 in plasma and PBMCs. There were significant differences on the expression of miR-155 in plasma and PBMCs, the apoptosis rate of PBMCs, Gensini score and the extent of coronary stenosis among the three groups (P0.05). The expression of miR-155 in plasma and PBMCs in AMI and AP group were lower than CT group while the AMI group was lower than the AP group (P0.05). The apoptotic rate of PBMCs, Gensini score and the extent of coronary stenosis of the AMI and AP groups were higher than CT group while the AMI group was higher than the AP group (P0.05). The expression of miR-155 in plasma was positively correlated with PBMCs, but negatively correlated with the apoptosis rate of PBMCs, Gensini score and the extent of coronary stenosis. The apoptosis rate of PBMCs in patients with coronary heart disease was positively correlated with the degree of coronary artery stenosis and Gensini score. In conclusion, in the patients with coronary heart disease the apoptosis rate of PBMCs was increased and the expression of miR-155 in plasma and PBMCs cells was decreased, which were correlated with the severity of coronary heart disease.
机译：该研究的目的是研究MiR-155在血浆和外周血单核细胞（PBMC）中的表达，miR-155对PBMC凋亡率以及冠状动脉疾病患者冠状动脉狭窄程度的影响。七十个胸痛患者按症状，症状，辅助检查和冠状动脉造影分为三组：21例急性心肌梗死组（AMI），昂热肺炎组（AP）中23例，对照组26例（ CT）。三组患者的外周血单核细胞分离为细胞培养物。进行膜蛋白V /碘化丙啶（PI）以分析细胞凋亡。 RT-QPCR用于评估血浆和PBMC中miR-155的表达水平。血浆和PBMC中miR-155表达的显着差异，PBMCs的凋亡率，Gensini评分以及三组冠状动脉狭窄程度（P <0.05）。在AMI和AP组中的血浆和PBMC中miR-155的表达低于CT组，而AMI基团低于AP基团（P <0.05）。 PBMC，Gensini评分和AMI和AP组冠状动脉狭窄程度的凋亡率高于CT组，而AMI基团高于AP基团（P <0.05）。 MiR-155在血浆中的表达与PBMC呈正相关，但与PBMC，Gensini评分和冠状动脉狭窄程度的凋亡率负相关。冠心病患者PBMC凋亡率与冠状动脉狭窄和Gensini评分呈正相关。总之，在冠心病患者中，PBMC的凋亡率增加，血浆和PBMCS细胞中miR-155的表达减少，与冠心病的严重程度相关。

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  • 来源
  • 作者

    Zhao Duo; Zhao Jing; Sun Jiangbin; Su Yanling; Jian Jinfeng; Ye Huaan; Lin Jiawang; Yang Zongda; Feng Jiatao; Wang Zhiping;

  • 作者单位

    Sun Yat Sen Univ Affiliated Hosp 1 Dept Cardiovasc Surg 58 Zhongshan Rd Guangzhou 510080;

    Sun Yat Sen Univ Peoples Hosp Foshan 1 Dept Cardiovasc Surg Affiliated Foshan Hosp 81 North;

    Guilin Med Univ Affiliated Hosp Dept Cardiovasc Surg Guilin 541001 Guangxi Peoples R China;

    Sun Yat Sen Univ Peoples Hosp Foshan 1 Dept Cardiovasc Surg Affiliated Foshan Hosp 81 North;

    Sun Yat Sen Univ Peoples Hosp Foshan 1 Dept Cardiovasc Surg Affiliated Foshan Hosp 81 North;

    Sun Yat Sen Univ Peoples Hosp Foshan 1 Dept Cardiovasc Surg Affiliated Foshan Hosp 81 North;

    Sun Yat Sen Univ Peoples Hosp Foshan 1 Dept Cardiovasc Surg Affiliated Foshan Hosp 81 North;

    Sun Yat Sen Univ Peoples Hosp Foshan 1 Dept Cardiovasc Surg Affiliated Foshan Hosp 81 North;

    Sun Yat Sen Univ Peoples Hosp Foshan 1 Dept Cardiovasc Surg Affiliated Foshan Hosp 81 North;

    Sun Yat Sen Univ Affiliated Hosp 1 Dept Cardiovasc Surg 58 Zhongshan Rd Guangzhou 510080;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 治疗学;
  • 关键词

    coronary heart disease; miR-155; peripheral blood mononuclear cells; apoptosis; coronary stenosis;

    机译：冠心病;miR-155;外周血单核细胞;细胞凋亡;冠状动脉狭窄;

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