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首页> 外文期刊>Evolution & development >Molecular cloning, characterization, and expression profiles of the Sox3Sox3 gene in Chinese loach Paramisgurnus dabryanusParamisgurnus dabryanus
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Molecular cloning, characterization, and expression profiles of the Sox3Sox3 gene in Chinese loach Paramisgurnus dabryanusParamisgurnus dabryanus

机译： sox3 sox3基因中的分子克隆，表征和表达分布在中文Loach paramisgurnus dabryanus Paramisgurnus dabryanus

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摘要

> A number of studies have established that in vertebrates, Sox3 is involved in a wide range of developmental processes, including sex differentiation and neurogenesis. However, the exact functions of the Sox3 gene have not been documented so far in teleosts. Here, we cloned the full length cDNA of Sox3 from the teleost fish, Paramisgurnus dabryanus , which we designated PdSox3 . Sequence analysis revealed that PdSox3 encodes a hydrophilic protein, and shares high homology with Sox3 in other species, ranging from mammals to fishes. Quantitative real‐time reverse transcription PCR, and in situ hybridization showed that PdSox3 is consistently expressed during embryogenesis, mainly localized in the developing central nervous system. Tissue distribution analyses revealed that PdSox3 is abundant in the adult brain, especially in particle cell layer. Furthermore, PdSox3 expression was higher in gonads, in primary spermatocyte cells, primary oocytes, and previtellogenic oocyte cells. All of these results suggest that PdSox3 plays an important role in early embryonic development, in particular the formation and development of the nervous system, and gonad development, similarly to other vertebrates. This is the first report describing Sox3 gene expression from this species, and the results are necessary to provide fundamental information on both the functional and evolutionary role of Sox3 across different species.
机译：
>许多研究已经确定，在脊椎动物中， SOX3 参与了各种发育过程，包括性别分化和神经发生。然而，到目前为止，尚未记录 SOX3 基因的确切功能。在这里，我们克隆了来自Textost鱼的 Sox3 的全长cDNA， Paramisgurnus dabryanus ，我们指定了 pdsox3 。序列分析显示， PdSOx3 编码亲水蛋白，并在其他物种中与 SOX3-I>分享高同源性，从哺乳动物到鱼类。定量实时逆转录PCR，并且原位杂交显示，在胚胎发生期间始终表达 PdSOx 3，主要是在显影中枢神经系统中局部。组织分布分析表明，在成年脑中，特别是在颗粒细胞层中的 PdSOx3。此外，在原发性精子细胞，初级卵母细胞和最终的卵母细胞细胞中，GONAD中的 PdSOx3 表达较高。所有这些结果表明 Pdsox3 在早期胚胎发育中起重要作用，特别是神经系统的形成和发展，以及Gonad开发，与其他脊椎动物类似。这是第一个描述来自该物种的 SOX3 基因表达的第一个报告，并且结果是提供关于在不同物种中 SOX3 的功能和进化作用的基本信息。

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  • 来源
    《Evolution & development》 |2018年第4期|共11页
  • 作者

    Xia Xiaohua; Huo Weiran; Wan Ruyan; Wang Peijin; Zhang Linxia; Chang Zhongjie;

  • 作者单位

    College of Life ScienceHenan Normal UniversityXinxiang Henan People's Republic of China;

    College of Life ScienceHenan Normal UniversityXinxiang Henan People's Republic of China;

    College of Life ScienceHenan Normal UniversityXinxiang Henan People's Republic of China;

    College of Life ScienceHenan Normal UniversityXinxiang Henan People's Republic of China;

    College of Life ScienceHenan Normal UniversityXinxiang Henan People's Republic of China;

    College of Life ScienceHenan Normal UniversityXinxiang Henan People's Republic of China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物科学;
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