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首页> 外文期刊>Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function >Cloning, characterization and promoter analysis of S-RNase gene promoter from Chinese pear (Pyrus pyrifolia)
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Cloning, characterization and promoter analysis of S-RNase gene promoter from Chinese pear (Pyrus pyrifolia)

机译：中国梨（Pyrus Pyrifolia）的S-RNase基因启动子克隆，表征及启动子分析

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摘要

The 5'-flanking region of the S 12-, S 13-, S 21-RNase with a length of 854bp, 1448bp and 1137bp were successfully isolated by TAIL-PCR from genomic DNA from 'Jinhua', 'Maogong' (Pyrus pyrifolia) and 'Yali' (Pyrus bretschneideri) genomic DNA. Sequence alignment and analysis of S 13-, S 12-, S 21-RNase gene promoter sequences with S 2-, S 3-, S 4-, S 5-RNase 5'-flanking sequences indicated that a homology region of about 240bp exists in the regions just upstream of the putative TATA boxes of the seven Chinese/Japanese pear S-RNase genes. Phylogenetic tree suggests that the homology region between the Chinese/Japanese pear and apple S-RNase gene promoter regions reflects the divergence of S-RNase gene was formed before the differentiation of subfamilies. Full length and a series of 5'-deletion fragments-GUS fusions were constructed and introduced into Arabidopsis thaliana plants. GUS activity were detected in S 12-pro-(1 to 5)-GUS-pBll01.2 transgenic pistils and progressively decreased from S 12-pro-1-GUS-pBI l01.2 to S 12-pro-5-GUS-pBll01.2. No GUS activity was detected in S 12-pro-6-GUS-pBll01.2 transgenic pistil and other tissues of non-transformants and all transgenic plants. The result suggested S 12-RNase promoter is pistil specific expression promoter.
机译：通过“金华”，'Maogong'（Pyrus Pyrifolia）从“金华”（Pyrus Pyrifolia）的基因组DNA成功地分离了S12，S13，S21-RNase的554bp，144bp和1137bp的554bp，1448bp和1137bp的侧翼区域。 ）和'yali'（pyrus bretschneideri）基因组DNA。 S 13-，S12，S 21-RNase基因启动子序列的序列对准和分析S 2-，S 3-，S 4-，S 5-RNase5-侧侧侧侧序列表明，约240bp的同源性区域存在于七种中/日梨S-RNase基因的推定塔塔盒上游的地区。系统发育树表明中/日梨和苹果S-RNase基因启动子区之间的同源区反映了在亚壳的分化之前形成了S-RNase基因的分歧。构建并引入了全长和一系列5'-删除碎片 - GUS融合并引入拟南芥植物。在S12-PRO-（1至5） - GUS-PBLL01.2转基因雌激酶中检测到GUS活性，并从S 12-PRO-1-GUS-PBI L01.2至S 12-PRO-5-GUS逐渐降低pbll01.2。在S12-Pro-6-GUS-PBLL01.2转基因雌蕊和非转化体和所有转基因植物的其他组织中检测到GUS活性。结果表明S12-RNase启动子是雌蕊特异性表达启动子。

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