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首页> 外文期刊>Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function >Genome-wide analysis and expression profiling of the GRF gene family in oilseed rape (Brassica napus L.)
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Genome-wide analysis and expression profiling of the GRF gene family in oilseed rape (Brassica napus L.)

机译：油菜籽油菜（Brassica Napus L.）GRF基因家族的基因组分析及表达谱

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摘要

Growth regulating-factors (GRFs) are plant-specific transcription factors that help regulate plant growth and development. Genome-wide identification and evolutionary analyses of GRF gene families have been performed in Arabidopsis thaliana, Zea mays, Oryza sativa, and Brassica rapa, but a comprehensive analysis of the GRF gene family in oilseed rape (Brassica napus) has not yet been reported. In the current study, we identified 35 members of the BnGRF family in B. napus. We analyzed the chromosomal distribution, phylogenetic relationships (Bayesian Inference and Neighbor Joining method), gene structures, and motifs of the BnGRF family members, as well as the cis-acting regulatory elements in their promoters. We also analyzed the expression patterns of 15 randomly selected BnGRF genes in various tissues and in plant varieties with different harvest indices and gibberellic acid (GA) responses. The expression levels of BnGRFs under GA treatment suggested the presence of possible negative feedback regulation. The evolutionary patterns and expression profiles of BnGRFs uncovered in this study increase our understanding of the important roles played by these genes in oilseed rape. (C) 2017 Published by Elsevier B.V.
机译：生长调节因子（GRF）是有助于调节植物生长和发育的植物特异性转录因子。 GRF基因家族的基因组鉴定和进化分析已在拟南芥中进行，Zea Mays，Oryza Sativa和Brassica Rapa进行，但尚未报告对油菜（Brassica Napus）的GRF基因家族的综合分析。在目前的研究中，我们确定了B. Napus的BNGRF家族的35名成员。我们分析了BNGRF家族成员的染色体分布，系统发育关系（贝叶斯推理和邻居加入方法），基因结构和主题，以及其启动子中的CIS作用调节元件。我们还分析了各种组织中15种随机选择的BNGRF基因的表达模式，以及不同的收获索引和凝胶酸（GA）反应的植物品种。 GA治疗下BNGRF的表达水平表明存在可能的负反馈调节。本研究中未发现的BNGRFS的进化模式和表达谱增加了我们对油菜中这些基因发挥的重要角色的理解。 （c）2017年由Elsevier B.V发布。

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