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首页> 外文期刊>Evidence-based complementary and alternative medicine: eCAM >Ethanol Extracts of Fruiting Bodies of Antrodia cinnamomea Suppress CL1-5 Human Lung Adenocarcinoma Cells Migration by Inhibiting Matrix Metalloproteinase-2/9 through ERK, JNK, p38, and PI3K/Akt Signaling Pathways
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Ethanol Extracts of Fruiting Bodies of Antrodia cinnamomea Suppress CL1-5 Human Lung Adenocarcinoma Cells Migration by Inhibiting Matrix Metalloproteinase-2/9 through ERK, JNK, p38, and PI3K/Akt Signaling Pathways

机译：通过ERK，JNK，P38和PI3K / AKT信号通路抑制基质金属蛋白酶-2 / 9，抑制CL1-5抑制CL1-5的乙醇提取物抑制CL1-5人肺腺癌细胞迁移

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Cancer metastasis is a primary cause of cancer death. Antrodia cinnamomea (A. cinnamomea), a medicinal mushroom in Taiwan, has shown antioxidant and anticancer activities. In this study, we first observed that ethanol extract of fruiting bodies of A. cinnamomea (EEAC) exerted a concentration-dependent inhibitory effect on migration and motility of the highly metastatic CL1-5 cells in the absence of cytotoxicity. The results of a gelatin zymography assay showed that A. cinnamomea suppressed the activities of matrix metalloproteinase-(MMP-) 2 and MMP-9 in a concentration-dependent manner. Western blot results demonstrated that treatment with A. cinnamomea decreased the expression of MMP-9 and MMP-2; while the expression of the endogenous inhibitors of these proteins, that is, tissue inhibitors of MMP (TIMP-1 and TIMP-2) increased. Further investigation revealed that A. cinnamomea suppressed the phosphorylation of ERK1/2, p38, and JNK1/2. A. cinnamomea also suppressed the expressions of PI3K and phosphorylation of Akt. Furthermore, treatment of CL1-5 cells with inhibitors specific for PI3K (LY 294002), ERK1/2 (PD98059), JNK (SP600125), and p38 MAPK (SB203580) decreased the expression of MMP-2 and MMP-9. This is the first paper confirming the antimigration activity of this potentially beneficial mushroom against human lung adenocarcinoma CL1-5 cancer cells.
机译：癌症转移是癌症死亡的主要原因。 Antrodia Cinnamomea（A. Cinnamomea）是台湾的药用蘑菇，都表现出抗氧化和抗癌活动。在这项研究中，首先观察到A.肉瘤（EEAC）的乙醇提取物的乙醇提取物在没有细胞毒性的情况下对高度转移性CL1-5细胞的迁移和动力进行浓度依赖性抑制作用。明胶酶谱测定的结果表明，A. Cinnamomea以浓度依赖性方式抑制了基质金属蛋白酶 - （MMP-）2和MMP-9的活性。 Western印迹结果表明，用A. Cinnamomea治疗降低了MMP-9和MMP-2的表达;虽然这些蛋白质的内源性抑制剂的表达，即MMP（TIMP-1和TIMP-2）的组织抑制剂增加。进一步调查显示，A.肉桂膜抑制了ERK1 / 2，P38和JNK1 / 2的磷酸化。 A. Cinnamomea还抑制了AKT的PI3K和磷酸化的表达。此外，对PI3K（LY 294002），ERK1 / 2（PD98059），JNK（SP600125）和P38MAPK（SB203580）的特异性特异性抑制剂的CL1-5细胞降低了MMP-2和MMP-9的表达。这是鉴于对人肺腺癌Cl1-5癌细胞的潜在有益蘑菇的抗偏移活性的第一篇论文。

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