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首页> 外文期刊>Evidence-based complementary and alternative medicine: eCAM >Mechanistic Study of the Phytocompound, 2-beta-D-Glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne in Human T-Cell Acute Lymphocytic Leukemia Cells by Using Combined Differential Proteomics and Bioinformatics Approaches
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Mechanistic Study of the Phytocompound, 2-beta-D-Glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne in Human T-Cell Acute Lymphocytic Leukemia Cells by Using Combined Differential Proteomics and Bioinformatics Approaches

机译：用组合鉴别蛋白质组学和生物信息学方法，使用组合蛋白质组和生物信息学方法研究植物中复杂，2-β-D-吡喃吡喃氧基氧基-1-羟基葡聚糖-5,7,9,11-四酮

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Bidens pilosa, a medicinal herb worldwide, is rich in bioactive polyynes. In this study, by using high resolution 2-dimensional gel electrophoresis coupled with mass spectrometry analysis, as many as 2000 protein spots could be detected and those whose expression was specifically up-or downregulated in Jurkat T cells responsive to the treatment with 2-beta-D-glucopyranosyloxy-1-hydroxytrideca-5,7,9,11-tetrayne (GHTT) can be identified. GHTT treatment can upregulate thirteen proteins involved in signal transduction, detoxification, metabolism, energy pathways, and channel transport in Jurkat cells. Nine proteins, that is, thioredoxin-like proteins, BH3 interacting domain death agonist (BID protein involving apoptosis), methylcrotonoyl-CoA carboxylase beta chain, and NADH-ubiquinone oxidoreductase, were downregulated in GHTT-treated Jurkat cells. Further, bioinformatics tool, Ingenuity software, was used to predict signaling pathways based on the data obtained from the differential proteomics approach. Two matched pathways, relevant to mitochondrial dysfunction and apoptosis, in Jurkat cells were inferred from the proteomics data. Biochemical analysis further verified both pathways involving GHTT in Jurkat cells. These findings do not merely prove the feasibility of combining proteomics and bioinformatics methods to identify cellular proteins as key players in response to the phytocompound in Jurkat cells but also establish the pathways of the proteins as the potential therapeutic targets of leukemia.
机译：Bidens Pilosa是全球药草，富含生物活性的多褐色。在该研究中，通过使用高分辨率二维凝胶电泳与质谱分析耦合，可以检测到多达2000个蛋白质点，并且在响应于2-β的治疗的治疗中，在Jurkat T细胞中特别升高的那些表达的那些可以鉴定-D-葡糖吡喃氧氧基氧基-1-羟基葡萄糖烷基-5,7,9,11-四苯（GHTT）。 GHTT治疗可以上调三术蛋白，参与Jurkat细胞中的信号转导，解毒，代谢，能量途径和通道运输。在GHTT治疗的Jurkat细胞中下调，下调九种蛋白质，即甲状腺素样蛋白，BH3相互作用的蛋白质，BH3相互作用的域死亡激动剂（涉及凋亡的BID蛋白），甲基丙酮酰基-COA羧化酶β链和NADH-泛烯酮氧化酶。此外，使用生物信息工具，聪明的软件，用于基于从差分蛋白质组学方法获得的数据来预测信令途径。从蛋白质组学数据推断使用与线粒体功能障碍和细胞凋亡相关的两种匹配途径。生物化学分析进一步验证了涉及Jurkat细胞的GHTT的途径。这些发现不仅可以证明组合蛋白质组学和生物信息学的可行性，以鉴定细胞蛋白作为关键球员，响应Jurkat细胞的植物植物，而且建立蛋白质的途径作为白血病的潜在治疗靶标。

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