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首页> 外文期刊>Experimental Thermal and Fluid Science: International Journal of Experimental Heat Transfer, Thermodynamics, and Fluid Mechanics >Microscale two-dimensional (2D) temperature mapping by ratiometric fluorescence imaging under orthogonal excitations
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Microscale two-dimensional (2D) temperature mapping by ratiometric fluorescence imaging under orthogonal excitations

机译：微观二维（2D）温度映射通过正交激励下的比率荧光成像

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摘要

Microscale temperature mapping in liquids is of great importance in many areas of research such as microfluidics and biology. Among the current thermometric approaches, optical probing using fluorescence is particularly desirable because of its high spatial resolution and non-invasive nature. Here we report a new microscale two-dimensional (2D) fluorescence thermometry. This method exploits the temperature dependence of rotational molecular motion and its influence on the depolarization of fluorescence light, by measuring the difference in fluorescence intensities excited by orthogonal polarizations. With one charge coupled device (CCD) camera, we get 2D ratiometric mappings of temperature from successively recorded fluorescence images under alternatively polarized excitations. We demonstrate reliable temperature mapping in liquids at sub-1 degrees C temperature accuracy and sub-10 mu m spatial resolution. We also show that the proposed thermometry approach is robust against fluorescence intensity variations, suitable for 2D mapping and of fast readout that is comparable to CCD framerate. Moreover, it is easy to be integrated into microscope systems since only rotation of excitation polarization is needed.
机译：液体中的微观温度测绘在许多研究领域，如微流体和生物学等许多研究领域都很重要。在当前的温度方法中，由于其高空间分辨率和非侵入性，因此使用荧光的光学探测是特别理想的。在这里，我们报告了一种新的微米二维（2D）荧光测温法。该方法利用旋转分子运动的温度依赖性及其对荧光去极化的影响，通过测量正交偏振激励的荧光强度差异。通过一个充电耦合器件（CCD）相机，我们将在替代地激发的连续记录荧光图像中获得2D比率映射。我们展示了Sub-1摄氏度的液体中的可靠温度映射和亚10μm空间分辨率。我们还表明，该拟议的测温方法对荧光强度变化具有鲁棒，适用于2D映射和与CCD帧相当的快速读数。此外，易于集成到显微镜系统中，因为只需要激励极化的旋转。

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