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首页> 外文期刊>BJOG: an international journal of obstetrics and gynaecology >Health professionals need to target exclusive breastfeeding.
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Health professionals need to target exclusive breastfeeding.

机译：卫生专业人员需要针对纯母乳喂养。

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摘要

Malaria parasites are under oxidative attack throughout their life cycle in human body and mosquito vector. Therefore, Plasmodium antioxidant defenses are crucial for its survival and being considered as interesting target for antimalarial drug design. Plasmodium knowlesi has emerged recently from its simian host to human in Southeast Asia and has been recognized as the fifth Plasmodium species that can cause human malaria. In this study, we cloned and characterized thioredoxin peroxidase 1 from P. knowlesi (PkTPx-1). PkTPx-1 gene was cloned, and recombinant protein was produced by heterologous overexpression in Escherichia coli. The recombinant protein was used for evaluation of enzymatic activity and polyclonal antibody production. Using the recombinant PkTPx-1 protein, its antioxidant activity was confirmed in a mixed-function oxidation assay where PkTPx-1 prevented nicking of DNA by hydroxyl radicals. PkTPx-1 was able to bind to double-strand DNA and RNA and had RNA chaperone activity in a nucleic acid melting assay indicating new function of PkTPx-1 other than antioxidant activity. Using specific polyclonal antibodies, it was indicated that PkTPx-1 is expressed in the cytoplasm of the parasite. Altogether, these results suggest that PkTPx-1 not only protects the parasite from the adverse effects of reactive oxygen species but also has RNA chaperone activity.
机译：疟原虫在人体和蚊子的整个生命周期中都受到氧化攻击。因此，疟原虫抗氧化剂防御对其生存至关重要，并被认为是抗疟疾药物设计的有趣目标。恶性疟原虫最近已从其猿猴宿主出现到东南亚的人类，并被公认为是可导致人类疟疾的第五种恶性疟原虫。在这项研究中，我们克隆并表征了诺氏疟原虫（PkTPx-1）的硫氧还蛋白过氧化物酶1。克隆PkTPx-1基因，并通过在大肠杆菌中的异源过表达产生重组蛋白。重组蛋白用于评估酶活性和多克隆抗体的产生。使用重组的PkTPx-1蛋白，在混合功能氧化分析中证实了其抗氧化活性，其中PkTPx-1阻止了羟基自由基对DNA的切割。 PkTPx-1能够与双链DNA和RNA结合，并在核酸熔解测定中具有RNA伴侣活性，表明PkTPx-1的新功能是抗氧化活性。使用特定的多克隆抗体，表明PkTPx-1在寄生虫的细胞质中表达。总而言之，这些结果表明PkTPx-1不仅可以保护寄生虫免受活性氧的不利影响，而且还具有RNA伴侣活性。

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