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首页> 外文期刊>Genetic Resources and Crop Evolution >Development of nuclear microsatellite markers to facilitate germplasm conservation and population genetics studies of five groups of tropical perennial plants with edible fruits and shoots: rambutan (Nephelium lappaceumL.), sapodilla (Manilkara zapota(L.) P. Royen), lychee (Litchi chinensisSonn.), mangosteen (Garcinia mangostanaLinn. andGarcinia cochinchinensis(Lour.) Choisy) and bamboo (Bambusa vulgarisSchrad. ex JC Wendl andGuadua angustifoliaKunth)
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Development of nuclear microsatellite markers to facilitate germplasm conservation and population genetics studies of five groups of tropical perennial plants with edible fruits and shoots: rambutan (Nephelium lappaceumL.), sapodilla (Manilkara zapota(L.) P. Royen), lychee (Litchi chinensisSonn.), mangosteen (Garcinia mangostanaLinn. andGarcinia cochinchinensis(Lour.) Choisy) and bamboo (Bambusa vulgarisSchrad. ex JC Wendl andGuadua angustifoliaKunth)

机译：核微卫星标志物的开发，以促进食用水果和芽五组热带多年生植物的种质养护和群体遗传研究：红霉（Nephelium Lappaceuml。），Sapodilla（Manilkara Zapota（L.）P. Royen），Lychee（Litchi Chinensisson 。），山竹（Garcinia Mangostanalnnn。Andgarcinia cochinchinensis（rour。）choisy）和bamboo（bambusa wulgarisschrad。前JC Wendl和Guadua Angustifoliakunth）

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Simple sequence repeat (SSR) enriched libraries for five groups of tropical perennial plants with edible fruits and shoots were prepared and sequenced in a GS-FLX Roche 454: sapodilla (Manilkara zapota(L.) P. Royen), lychee (Litchi chinensisSonn.), mangosteen (Garcinia mangostanaLinn. andG. cochinchinensis(Lour.) Choisy), rambutan (Nephelium lappaceumL.), and bamboo (Bambusa vulgarisSchrad. ex J.C. Wendl andGuadua angustifoliaKunth). For SSR development, these species were organized by their common names in five groups. A total of 3870 SSR primer sets were designed, using capillary electrophoresis 1872 nuclear SSRs were tested on 4 to 10 DNA samples within each plant group, that is 384 loci for each of the four groups of fruit trees and 336 loci for the bamboo group. Only 7.9% of the primers tested did not result in amplification. All 1872 SSRs are provided, we highlight 178 SSRs (between 26 and 47 per group) considered top-quality polymorphic SSRs that amplified all the samples, had strong fluorescence signal, presented no stutters and showed minimum non-specific amplification or background fluorescence. A total of 66,057 contig sequences were submitted to GenBank Database. Markers presented here will be useful not only for conservation efforts in banks of germplasm, but also for in-depth analysis of population genetics which usually requires evaluation of large number of loci.
机译：在GS-FLX Roche 454：Sapodilla（Manilkara Zapota（L.）P. Royen），Lychee（Litchi Chinensiss），制备简单的序列重复（SSR）具有可食用水果和芽的五组热带多年生植物的文库。 ），Mangosteen（Garcinia mangostanalinn。Cochinchinensis（Lour。）choisy），红毛丹（Nephelium Lappaceuml。）和竹子（Bambusa Vulgarisschrad。前JC Wendl和Guadua Angustifoliakunth）。对于SSR开发，这些物种由他们的共同名称组织在五组中。设计了总共3870个SSR引物套，使用毛细管电泳1872核SSRS在每种植物组中的4至10个DNA样品中测试，为四组果树和336个竹组基因座中的384个基因座。只测试的7.9％的引物未导致扩增。提供所有1872个SSRS，我们突出显示178个SSRS（每组26到47个）被认为是放大所有样品的高质量多态性SSRS，具有强大的荧光信号，没有刻表，显示出最小的非特异性扩增或背景荧光。总共提交了66,057种Centig序列到Genbank数据库。这里提出的标记对于种质银行的保护努力，而且还用于对人口遗传学的深入分析，这通常需要评估大量基因座。

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