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Collection of new diversity of wild and cultivated bananas (Musa spp.) in the Autonomous Region of Bougainville, Papua New Guinea

机译:野生栽培香蕉新多样性的集合(Musa SPP。)在巴布亚新几内亚九重葛自治区

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摘要

Bananas (Musa spp.), including dessert and cooking types, are of major importance in the tropics. Due to extremely high levels of sterility, the diversity of cultivated bananas is fixed over long periods of time to the existing genotypes. This pattern puts banana-based agrosystems at risk. Therefore, assessing the extent of wild and cultivated banana diversity, conserving it and making it available for further use is a priority. We report here the collection of new wild and cultivated banana germplasm in the Autonomous Region of Bougainville, Papua New Guinea. In total, 61 accessions were collected and their names and uses were recorded when possible. Classification was also provided based on the observations made in the field. Three wild specimens were collected. Among the 58 cultivated accessions, we noted that eight were used as ornamental plants, seven were edible varieties of the Fe'i type and two were natural tetraploids from the Musa section. The ploidy was then checked by flow cytometry and the accessions were genotyped with a set of 19 SSR markers. The genotyping results were merged to the dataset from Christelova et al. (Biodivers Conserv 26:801-824, 2017). This joint analysis helped refine or confirm the classification of the collected accessions. It also allowed to identify 10 private alleles and 35 genotypes or Genotype Groups that were not present in the wider dataset. Finally, it shed light on the diversification processes at work in the region, such as the capture of mutations by farmers and the likely occurrence of geneflow within the cultivated genepool.
机译:香蕉(Musa SPP),包括甜点和烹饪类型,在热带地区具有重要意义。由于耐受性极高,栽培香蕉的多样性在长时间内固定到现有基因型。这种模式将香蕉基的农业系统造成风险。因此,评估野生和培养的香蕉多样性,保护它并使其可用于进一步使用的程度优先。我们在此报告在巴布亚新几内亚九重葛自治区新野生和栽培香蕉种质的集合。总共收集了61种附加,并在可能的情况下记录其名称和用途。还根据现场的观察结果提供分类。收集了三种野生标本。在58种培养的过程中,我们指出,八种被用作观赏植物,七种是Fe'i类型的食用品种,两种是来自Musa段的天然四倍体。然后通过流式细胞术检查倍增倍性,并用一组19个SSR标记进行缩进。基因分型结果与Christelova等人的数据集合并。 (生物方向保证26:801-824,2017)。该联合分析有助于改进或确认所收集的换乘的分类。它还允许鉴定10个私人等位基因和35个基因型或基因型组,其不存在于更宽的数据集中。最后,它在该地区工作中的多样化过程中的光线阐明,例如农民捕获的突变以及培养的基因内的谷胱甘肽的可能发生。

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