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首页> 外文期刊>Genetic Resources and Crop Evolution >Microsatellite markers in Spanish lime (Melicoccus bijugatus Jacq., Sapindaceae), a neglected Neotropical fruit crop
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Microsatellite markers in Spanish lime (Melicoccus bijugatus Jacq., Sapindaceae), a neglected Neotropical fruit crop

机译：西班牙石灰的微卫星标记（Melicoccus bijugatus jacq。，sapindaceae），被忽视的新生儿果实

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摘要

Spanish lime (Melicoccus bijugatus Jacq.) is a Neotropical fruit tree cultivated, mainly, in orchards for self-consumption or local sale. The genus Melicoccus includes other nine species with edible fruits, some of these species are at risk of extinction. Like for the vast majority of tropical fruit trees, there is no information on the genetic diversity of Spanish lime and its related species, and this is mostly due to the lack of molecular markers. The objectives of this study were to present the first microsatellite markers developed for Spanish lime, testing its usefulness on a sample of cultivated accessions, as well as its transferability to Huaya India (M. oliviformis). To do this, we performed high-throughput sequencing of microsatellite-enriched libraries of Spanish lime using Roche 454, assembled 9567 DNA contig sequences and identified 10,117 microsatellites. After screening 384 of those microsatellites on four DNA samples, 31 polymorphic markers were used to screen 25 accessions of Spanish lime and five of Huaya India collected in Yucatan, Mexico. Genetic diversity was low in Spanish lime (A = 20.61, H-E = 0.38) and similar for both sexes of this species. Neighbor-Joining and PCoA analyses clearly discriminated between the two Melicoccus species studied. Nine of the markers showed unique alleles for Huaya India. The set of microsatellite markers developed has a great potential to generate information in relation to conservation genetics, improvement of elite cultivars and breeding programs for Spanish lime and related species.
机译：西班牙石灰（Melicoccus bijugatus jacq。）是一种培养的新果树，主要是在果园的自我消费或地方销售。 Melicoccus属包括其他九种具有可食用果实的物种，其中一些物种面临灭绝的风险。喜欢绝大多数热带果树，没有关于西班牙石灰和其相关物种的遗传多样性的信息，这主要是由于缺乏分子标记。本研究的目的是介绍为西班牙石灰开发的第一个微卫星标志物，测试其对栽培种植样本的用途，以及其对华纳印度（M. Oliviformis）的可转移性。为此，我们使用Roche 454对西班牙石灰的微卫星富集的文库进行了高通量测序，组装了9567DNA Contig序列并鉴定了10,117微卫星。在四个DNA样品上筛选那些微卫星的384时，31种多态性标记物用于筛选西班牙石灰的25种，在墨西哥尤卡坦收集的华纳印度五个。西班牙石灰（A = 20.61，H-E = 0.38）的遗传多样性低，并且对于这种物种的性别类似。邻近的和PCOA分析明确区分了所研究的两种血糖物种。九个标记显示了华纳印度的独特等位基因。该组微卫星标记产生了巨大的潜力，可以产生与保护遗传学，改善西班牙石灰和相关物种的精英品种和育种计划的信息。

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