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Genetic diversity of Ethiopian Xanthosoma sagittifolium (L.) Schott accessions assessed with AFLPs

机译:埃塞俄比亚黄酮瘤梭痘遗传多样性(L.)Schott accive评估AFLPS评估

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Xanthosoma sagittifolium (L.) Schott originated from the American tropics. Domestication may have occurred in various places as this Araceae species is an important food source. It has been cultivated for many decades. In this study, Amplified Fragments Length Polymorphism (AFLP) markers were used to analyze the genetic relationships among 78 Ethiopian X. sagittifolium accessions, for conservation purpose. Cormels were collected from Bench-Maji, Kefa, Dawuro and Wolaita zones, representing four populations. The accessions belonged to either green (G) or purple (P) colored leaf and petiole accessions. Three different AFLP primer combinations resulted in 478 scorable bands, of which 99.2% were polymorphic. The mean Nei's gene diversity (He) within populations was 0.35 while the G accessions featured higher He (0.38) than the P ones (0.35). The Nei's gene diversity (He) at entire collection level was 0.38. The detected high genetic diversity may indicate the X. sagittifolium plants growing in the country may derive from diverse parental genotype stock elsewhere and/or there may be multiple introductions to the country. Low levels of genetic differentiation were detected among populations (Gst=0.07) and between the G and P accessions (Gst=0.02). Insignificant genetic and geographic correlation was revealed by Mantel test. Clustering analysis grouped 91% of the accessions together. Conservation and management of X. sagittifolium in the country should concentrate on maintaining high level genetic diversity within each population as well as at entire collection level through both ex situ and in situ conservation actions.
机译:Xanthosoma Sagittifolium(L.)Schott起源于美国热带地区。由于这种橡木物种是一个重要的食物来源,在各个地方可能已经发生了驯化。它已经耕种了数十年。在该研究中,扩增的碎片长度多态性(AFLP)标记用于分析78个埃塞俄比亚X. Sagitifolium寻求庇护者的遗传关系,用于保护目的。从Bench-Maji,Kefa,Dawuro和Wolaita区收集鸬鹚,代表四个人口。附加属于绿色(g)或紫色(p)彩色叶子和叶柄acadions。三种不同的AFLP引物组合导致478个可批量的带,其中99.2%是多态的。群体内的平均内部的基因多样性(HE)为0.35,而G加入较高的G HE(0.38)(0.35)。整个收集水平的Nei的基因多样性(HE)为0.38。检测到的高遗传多样性可能表明该国生长的X. Sagitifolium植物可能来自其他地方的不同的父母基因型库存和/或可能对该国进行多次介绍。在群体(GST = 0.07)中检测到低水平的遗传分化,并在G和P附加(GST = 0.02)之间。 Mantel试验揭示了微不足道的遗传和地理相关性。聚类分析将91%的换乘器组合在一起。该国X. Sagitifolium的保护和管理应专注于维持每个人口内的高水平遗传多样性,以及通过以外的原地和原位保护行动的整个收集水平。

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