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首页> 外文期刊>Genetic Resources and Crop Evolution >Seeds capture the diversity of genetic resource collections of Malus sieversii maintained in an orchard
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Seeds capture the diversity of genetic resource collections of Malus sieversii maintained in an orchard

机译：种子捕获Malus Sieversii的遗传资源收集的多样性维持在果园里

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Many species in genebank collections are maintained vegetatively either as in vitro cultures or as plants in the field or greenhouse. In these cases, individual genotypes (cultivars) are the focus of conservation. In crop wild relatives, where the value of an accession is in the heterogeneity of segregating genotypes, seeds may be the most representative form of conservation. We used two sets of field-planted Malus sieversii apple trees that originated from seeds collected from two wild populations in Kazakhstan as a model system to determine if seeds from hand-pollinated crosses could be used to represent the allelic diversity of the parent trees in a genebank orchard. A crossing design was developed that used each of the selected trees from the two Kazakhstan collection sites as mothers and also as fathers in the form of bulked pollen pools. The sets of offspring from these crosses were genotyped using microsatellite markers. Paternity analyses revealed the percentage of offspring that was derived from each of the parent trees. We also determined the contribution of each parent to the fertilization events from the pollen pools. Although there were some unintended pollination events (fertilization by trees in unexpected pollen pools) and unequal contribution of pollen parents within pollen pools, we demonstrated that the seedlings effectively captured the allelic frequency and diversity of the parents. A seed collection strategy is the most efficient way to conserve the diversity of crop wild relatives even in collections typically conserved as individual clones.
机译：基因库收集中的许多物种都是在体外培养物中或植物或温室的植物维持。在这些情况下，个体基因型（品种）是保护的重点。在农作物野生亲属中，加入的价值处于隔离基因型的异质性，种子可能是最具代表性的保护形式。我们使用两组田间种植的Malus Sieversii苹果树，该树木源于从哈萨克斯坦的两个野生种群收集的种子，作为模型系统，以确定来自手工授粉十字架的种子可用于代表父母树的等位基因多样性Genebank果园。开发了一种穿越设计，这些设计将每个选定的树木从两个哈萨克斯坦收集地点的每个选定的树木作为母亲，也是祖先的花粉池的形式。使用微卫星标记物的这些十字架的后代的套装是基因分型。父权分析揭示了从每个母树中得出的后代的百分比。我们还确定了每个父母对花粉池的施肥事件的贡献。虽然有一些意外的授粉事件（在意外花粉池中的施肥），但花粉父母在花粉池中的不等贡献，我们证明幼苗有效地捕获了父母的等位基因频率和多样性。种子收集策略是即使在通常被保守为单个克隆的收藏中，也是节省农作物野生亲属多样性的最有效方式。

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