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首页> 外文期刊>Genetic Resources and Crop Evolution >High proportion of diploid hybrids produced by interspecific diploid x tetraploid Sorghum hybridization
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High proportion of diploid hybrids produced by interspecific diploid x tetraploid Sorghum hybridization

机译：间隙二倍体x四倍体高粱杂交产生的高比例二倍体杂交种

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A perennial version of grain sorghum [S. bicolor (L.) Moench] would create opportunities for greatly reducing tillage and preventing soil degradation. Efforts to select for perenniality and grain production among progeny of hybrids between S. bicolor (2n = 20) and the weedy tetraploid perennial S. halepense (L.) Pers. (2n = 40) are complicated in that F-1 hybrids produced by diploid x tetraploid sorghum crosses are usually tetraploid. In 2013, a set of random pollinations between 19 diploid cytoplasmic male-sterile inbred lines and 43 tetraploid perennial plants produced 165 F-1 hybrid plants, more than 75% of which had highly atypical plant, panicle, and seed phenotypes. Phenotypic segregation in F-2 populations derived from atypical hybrids was also anomalous. Examination of mitotic metaphase cells in F-1 or F-2 root tips revealed that 129 of the 165 hybrids were diploid. Parentage of the diploid progenies was confirmed using simple-sequence repeat analysis. The mechanism by which diploid hybrids arise from diploid x tetraploid crosses is unknown, but it may involve either production of monohaploid (n = 10) pollen by the tetraploid parent or chromosome elimination during early cell divisions following formation of the triploid zygote. The ability to produce diploid germplasm segregating for S. bicolor and S. halepense alleles could have great utility, both for the development of perennial sorghum and for the improvement of conventional grain sorghum.
机译：粮食高粱的多年生植物版[S.双色（L.）Moench]将大大降低耕作和防止土壤退化的机会。努力选择常年性和谷物产量的杂交种与杂交种之间的汞（2n = 20）和杂草四倍体多年生S. halepense（L.）pers。 （2N = 40）在二倍体x四倍体高粱交叉中产生的F-1杂种通常是四倍体的。 2013年，一组随机授粉在19个二倍体细胞质雄性无菌近交系和43种四倍体多年生植物之间产生了165件F-1杂交植物，其中75％以上具有高度的非典型植物，穗和种子表型。来自非典型杂交种群的F-2种群中的表型偏析也异常。在F-1或F-2根提示中检测有丝分裂中期细胞显示，165个杂种的129个是二倍体。使用简单序列重复分析确认二倍体后代的父母。二倍体x四倍体杂交出现的机制是未知的，但是在形成三倍体基因克特后，它可能涉及通过四倍体父母或染色体消除的单倍体（n = 10）花粉的生产。生产二倍体种质的能力为双子石和S.六粒子等位基因可以具有很大的效用，用于常年高粱的开发和改善常规谷物高粱。

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