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首页> 外文期刊>Genetic Resources and Crop Evolution >Genetic distinctiveness of a Protected Geographic Indication lentil landrace from the Umbria region, Italy, over 20 years
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Genetic distinctiveness of a Protected Geographic Indication lentil landrace from the Umbria region, Italy, over 20 years

机译：来自意大利翁布里亚地区的受保护地理指示扁片地球队的遗传独特性，超过20年

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摘要

Traceability of local and typical food products is essential for market protection and consumer trust. DNA analysis is the most dependable tool to identify the biological materials present in a food product. In this work, we molecularly characterized an important local product of the Umbria region, a lentil landrace named after the village Castelluccio di Norcia, in the province of Perugia, Italy, awarded the Protected Geographic Indication (PGI) "Lenticchia di Castelluccio di Norcia" by the European Union in 1997. Four accessions from a 1994 collection were compared with accessions acquired in 2016 at local retailers, in comparison with two commercial samples from grocery stores and two foreign varieties. Twelve simple sequence repeat (SSR) molecular markers allowed to clearly establish the genetic distinctiveness of local lentil. High allelic diversity and very low heterozygosity were observed in Castelluccio samples. The 1994 materials were closely related, as shown by pairwise Fst and by Principal Coordinates Analysis. Little divergence between 1994 and 2016 samples was found, possibly due to 20 years of cultivation in different farms, but one of the 2016 samples was unrelated, as were the two commercial samples. The genetic identity of the Castelluccio landrace is still preserved locally, but may be put at risk by the presence on the market of unrelated materials labeled as PGI. In Castelluccio accessions, as many as 42 private SSR alleles were documented, which can be implemented to develop molecular traceability tests.
机译：局部和典型食品的可追溯性对于市场保护和消费者信任至关重要。 DNA分析是最可靠的工具，用于识别食品中存在的生物材料。在这项工作中，我们分子特征在于翁布里亚地区的重要本地产品，这是一位以意大利佩鲁贾省Castelluccio di Norcia命名的扁豆地区，授予受保护地理指示（PGI）“Lenticchia di Castelluccio di Norcia” 1997年由欧洲联盟。1994年收集的四次加入与2016年在当地零售商收购的加入，与来自杂货店和两个异物的两种商业样本相比。 12个简单的序列重复（SSR）分子标记允许明确建立当地扁豆的遗传性质。在Castelluccio样品中观察到高位等多样性和非常低的杂合子。 1994年材料密切相关，如对FST和通过主坐标分析所示。 1994年至2016年样品之间的分歧很少，可能是由于不同农场的20年的培养，但2016样本之一是无关的，两种商业样本也是如此。 Castelluccio Landrace的遗传身份仍然在本地保存，但可以通过标记为PGI的无关材料市场的存在。在Castelluccio附加过程中，记录了多达42个私人SSR等位基因，可以实施以发展分子可追溯性测试。

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