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首页> 外文期刊>Genetic Resources and Crop Evolution >Evaluation of chemo-diversity in major Piper species for three piperamides using validated RP-HPLC method
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Evaluation of chemo-diversity in major Piper species for three piperamides using validated RP-HPLC method

机译：使用验证RP-HPLC方法评估三个哌酰胺主要吹笛剂物种的化学多样性

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摘要

India boasts rich biodiversity of plants belongs to Piperaceae. These plants are store-house of biologically active alkaloids, generally termed as piperamides. Among piperamides, piperine, piperlongumine and piperlonguminine plays an effective role in pharmaceutical applications. The present study was aimed at developing and validating a simple reverse phase high performance liquid chromatography method to analyze chemo-diversity of these three piperamides in Pipernigrum L., Piper longum L., Piper thomsonii Hook, Piper sarmentosum Roxb. and Piper chaba Trel. & Yunck. The separation was achieved on a RP-C18 column with water (0.1% Acetic acid) and methanol as mobile phase with detection wavelength of 338 nm. The compounds were identified using authentic standards. The developed method was validated as per ICH guidelines for linearity, accuracy, precision, limit of detection and limit of quantification. The method was successfully applied in quantification of these piperamides in berries of five different Piper species. Among them, piperlongumine was detected only in P. longum and P. sarmentosum. Piperlonguminine and piperine was higher in P. chaba and P. nigrum. In conclusion, the method developed could be used for chemo-diversity analysis of different Piper spp to select alternate Piper species for pharmacological preparations based on the composition of its bio-active compounds.
机译：印度拥有丰富的植物生物多样性属于Piperaceae。这些植物是生物活性生物碱的储存室，通常称为哌啶。在哌酰胺，哌啶，哌啶和哌啶胺在药物应用中起着有效作用。本研究旨在开发和验证一种简单的逆相高性能液相色谱法，用于分析Pipernigrum L.，Piper Longum L.，Piper Sarmentosum RoxB中这三个哌啶的化疗。和吹笛者chaba trel。 ＆amp; yunck。在具有水（0.1％乙酸）和甲醇的RP-C18柱上以具有338nm的波长的流动相，在RP-C18柱上实现分离。使用真实标准鉴定化合物。根据ICH线性，准确性，精度，检测限制和量化限制的指南验证了开发的方法。该方法以5种不同吹笛剂物种的浆果的这些哌酰胺的定量成功地应用。其中，仅在P. Longum和P. Sarmentosum中检测到Piperlongumine。 p. chaba和p. nigrum的piperlonguminine和pilerine较高。总之，开发的方法可用于不同吹笛器SPP的化疗分析，基于其生物活性化合物的组成，为药理学制剂选择交替吹笛剂物种。

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