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Transient albino and revertible to green (TARGreen)rice mutant: simple, affordable and beneficial novel tag for rapid genetic purity testing in rice (Oryza sativaL.)

机译:瞬态白果和绿色(targreen)米突变体(Targreen)米突变体:在水稻中快速遗传纯度试验(Oryza Sativalivalive)简单,价格实惠和有益的新标签。

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摘要

A key problem that long remains challenging in rice ecosystem restoration is how to ensure quality seeds to re-establish rice varietal specific communities. Despite the available approaches such as use of seeds from authenticated sources, it is invariably argued that such seeds may harbour little genetic variability. In this study, a simple, affordable and alternative strategy is proposed: use of mutant linetransient albino and revertible to green (TARGreen)derived fromOryza sativavar. ASD16. Rice line ASD16 is considered as a mega-variety in South India and it has shown rich harvest since its release in varying environments. However, the chief problem faced by the farmers is identification of genetically pure ASD16 plants in the field since it is often mixed with seeds from other rice-lines. To circumvent this problem, a chemical induced mutagenesis study was conducted in ASD16, which lead to the identification ofTARGreen. After germination,TARGreenremains albino for 21 days and after that, they gradually turn into green leaves. Further studies on this line over several generations established thatTARGreentrait was a Mendelian single recessive gene and the plants can able to produce the same quantity and quality of seeds as that of ASD16. Besides discussing immediate applications ofTARGreenin seed industry, its proposed applications in hybrid seed production and identification ofTARGreenspecific markers for its efficient introgression into other rice mega-varieties is also outlined hereunder.
机译:在水稻生态系统恢复中长期仍然具有挑战性的关键问题是如何确保优质种子重新建立水稻品种特定社区。尽管有可用的方法,如从经过身份验证的来源使用种子,但它总是认为这种种子可能含有很少的遗传变异性。在这项研究中,提出了一种简单,实惠且替代的策略:使用突变的线离子白果和可重复于绿色(targreen)衍生的FOFORYZA Sativavar。 ASD16。米线ASD16被认为是南印度的兆瓦品种,因为它在不同环境中释放以来,它表现出丰富的收获。然而,农民面临的主要问题是鉴定该领域的遗传纯度ASD16植物,因为它通常与来自其他稻米的种子混合。为了避免这个问题,在ASD16进行化学诱导的诱变研究,这导致鉴定鉴定的鉴定。发芽后,TargreenRemains Albino 21天后,它们逐渐变成绿叶。关于这一线的进一步研究在几代内建立了Thetargreentrait是孟德尔单一隐性基因,并且植物可以生产与ASD16的种子相同的种子和质量。除了讨论特达林素种子产业的直接应用外,还概述了其在杂交种子生产和鉴定中的杂交种子生产和鉴定的应用,以其有效的速度进入其他稻米品种。

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