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Analysis of genetic diversity and population structure in Saharan maize (Zea mays L.) populations using phenotypic traits and SSR markers

机译:撒哈拉玉米遗传多样性和人口结构分析,使用表型特征和SSR标记群体群体群体

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摘要

Algerian maize has been cultivated in Saharan Oases for many centuries, determining its adaption to extreme environments. Therefore, maize landraces from Sahara could be considered as valuable genetic resources for breeding. Morphological and molecular characterization of fifty-six populations were assessed using 14 agro-morphological traits and 18 SSR markers. Populations were evaluated in field experiment in an augmented randomized complete block design. ANOVA on morphological data revealed significant difference among populations. Analysis of principal component showed two principal components describing 55.44% of total variation. Flowering time, plant height, ears traits and yield were the most discriminatory traits. Genetic analysis identified a large number of alleles (191) with mean value of 10.61 alleles per locus. High average PIC value (0.57) indicates informativeness of the selected markers in this study. The genetic structure analysis revealed a high genetic differentiation (Fst=0.22) among populations, showing a greater genetic diversity within Algerian populations than among them. Bayesian model-based structure analysis assigned genotypes into two groups. Both phenotypic and SSR analysis revealed significant genetic diversity; albeit a clustering based on geographic origin was not observed. The wide genetic diversity of Saharan maize populations could be used as genetic resources in future maize breeding programs.
机译:阿尔及利亚玉米在撒哈拉果岭中培养了多个世纪,确定其适应极端环境。因此,来自撒哈拉州的玉米实地占据育种的宝贵遗传资源。使用14种农业形态学性状和18个SSR标记评估五十六种群体的形态学和分子表征。在增强随机完整块设计中,在现场实验中评估了种群。形态学数据的ANOVA在种群中显示出显着差异。主成分的分析显示了两个主要成分,描述了总变异的55.44%。开花时间,植物高度,耳朵性状和产量是最具歧视性的特征。遗传分析确定了大量的等位基因(191),平均值为10.61等位基因。高平均照片(0.57)表示本研究中所选标志的信息。遗传结构分析显示群体中高遗传分化(FST = 0.22),在阿尔及利亚人群中显示出比其中更大的遗传多样性。基于贝叶斯模型的结构分析将基因型分为两组。表型和SSR分析均显示出显着的遗传多样性;尽管未观察到基于地理原点的聚类。撒哈拉玉米种群的广泛遗传多样性可用于未来玉米育种计划中的遗传资源。

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