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首页> 外文期刊>Genetic Resources and Crop Evolution >Genetic diversity, population structure and identification of Dendrobium cultivars with high polysaccharide contents using SCoT, SCAR and nested PCR markers
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Genetic diversity, population structure and identification of Dendrobium cultivars with high polysaccharide contents using SCoT, SCAR and nested PCR markers

机译：苏格兰，瘢痕和巢式PCR标记的遗传多样性，人口结构和石斛品种鉴定

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摘要

Dendrobium officinale Kimura et Migo (D. officinale) is not only an ornamental plant with beautiful flowers but also a medicinal herb that is widely used in traditional Chinese medicine. We used start codon targeted (SCoT) polymorphism molecular markers to analyse eleven Dendrobium cultivars. Using 13 selected SCoT primers, 181 bands were generated, 157 (86.86%) of which were polymorphic. The eleven cultivars were clustered into two major groups based on a SCoT analysis by the unweighted pair-group method with arithmetic mean. Then, we developed SCoT-sequence-characterised amplified region (SCoT-SCAR) markers and SCoT-nested polymerase chain reactions as the complementary tools for the identification of high-polysaccharide-content D. officinale cultivars. A D. officinale Ruishen No.2'-specific band was detected in the PCR products. The primers, which were designed to target a specific band, amplified the target bands in D. officinale Ruishen No.2', but not in the ten other cultivars. The universal verification test showed that there were not any target bands being amplified with the primer SC1, SC2, and SC3 to the seventeen Dendrobium cultivars from all over the world, which more to prove the reliability of the experimental results using the SCoT-SCAR markers and the SCoT-nested PCR markers. This study provides guidelines for D. officinale Ruishen No.2' identification, for the breeding of D. officinale plants with a high content of active ingredients, and for industrial production.
机译：Dendrobium Officinale Kimura et Migo（D. Officinale）不仅是一种带有美丽花卉的装饰植物，而且是一种被广泛用于中医的药草。我们使用起始密码子靶向（SCOT）多态性分子标记分析11种石斛品种。使用13个选定的突出引物，产生181个带，其中157（86.86％）是多态的。基于算术平均值的未加权对 - 组方法基于苏格兰分析，将十一个品种聚集成两个主要群体。然后，我们开发了苏格兰序列表征表征的扩增区域（苏格兰疤痕）标记和苏格兰嵌套聚合酶链反应作为鉴定高多糖含量D. Officinale品种的互补工具。在PCR产物中检测到D. Officinale Ruishen No.2'特异性带。设计用于靶向特定带的引物扩增D. Officinale Ruishen No.2'中的目标带，但不在其他十种品种中。通用验证测试表明，没有用底漆SC1，SC2和来自世界各地的17个石斛品种的靶带扩增任何目标条带，这更多是使用苏格兰疤痕标记来证明实验结果的可靠性和苏格兰嵌套的PCR标记。本研究为D. Officinale Ruishen No.2'识别提供了指导性，用于培育具有高含量的活性成分，以及工业生产。

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  • 作者

    Li Qingguo; Mo Jianmei; Wu Wenru; Yang Juan; Li Jinjin; Lai Tianxing; Ou Zhanlun; Qiu Zhenwen; Guan Shixia; Liao Junjie;

  • 作者单位

    Guangzhou Univ Chinese Med Sch Pharmaceut Sci 232 Univ City Ring Rd East Guangzhou 510006 Guangdong Peoples R China;

    Guangzhou Univ Chinese Med Sch Pharmaceut Sci 232 Univ City Ring Rd East Guangzhou 510006 Guangdong Peoples R China;

    Guangzhou Univ Chinese Med Sch Pharmaceut Sci 232 Univ City Ring Rd East Guangzhou 510006 Guangdong Peoples R China;

    Guangzhou Univ Chinese Med Sch Pharmaceut Sci 232 Univ City Ring Rd East Guangzhou 510006 Guangdong Peoples R China;

    Guangdong Ind Tech Coll Dept Ecol &

    Environm Technol 152 Xingang Rd West Guangzhou 510300 Guangdong Peoples R China;

    Guangzhou Univ Chinese Med Sch Pharmaceut Sci 232 Univ City Ring Rd East Guangzhou 510006 Guangdong Peoples R China;

    Guangzhou Univ Chinese Med Sch Pharmaceut Sci 232 Univ City Ring Rd East Guangzhou 510006 Guangdong Peoples R China;

    Guangzhou Univ Chinese Med Clin Med Coll 1 Guangzhou 510006 Guangdong Peoples R China;

    Guangzhou Univ Chinese Med Sch Pharmaceut Sci 232 Univ City Ring Rd East Guangzhou 510006 Guangdong Peoples R China;

    Guangdong Ind Tech Coll Dept Ecol &

    Environm Technol 152 Xingang Rd West Guangzhou 510300 Guangdong Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 农业生物学;
  • 关键词

    Dendrobium officinale; SCoT marker; Genetic diversity; SCAR; Nested PCR;

    机译：Dendrobium Officinale;苏格兰标记;遗传多样性;疤痕;嵌套PCR;

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