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首页> 外文期刊>Genes to cells : >Chromatin tethering to the nuclear envelope by nuclear actin filaments: a novel role of the actin cytoskeleton in the Xenopus blastula
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Chromatin tethering to the nuclear envelope by nuclear actin filaments: a novel role of the actin cytoskeleton in the Xenopus blastula

机译：核肌动蛋白长丝的染色蛋白束缚到核信封：肌动蛋白细胞骨架在Xenopus Blastula中的一个新作用

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摘要

The Xenopus oocyte is known to accumulate filamentous or F-actin in the nucleus, but it is currently unknown whether F-actin also accumulates in embryo nuclei. Using fluorescence-labeled actin reporters, we examined the actin distribution in Xenopus embryonic cells and found that F-actin accumulates in nuclei during the blastula stage but not during the gastrula stage. To further investigate nuclear F-actin, we devised a Xenopus egg extract that reproduces the formation of nuclei in which F-actin accumulates. Using this extract, we found that F-actin accumulates primarily at the subnuclear membranous region and is essential to maintain chromatin binding to the nuclear envelope in well-developed nuclei. We also provide evidence that nuclear F-actin increases the structural stability of nuclei and contributes to chromosome alignment on the mitotic spindle at the following M phase. These results suggest the physiological importance of nuclear F-actin accumulation in rapidly dividing large Xenopus blastula cells.
机译：众所周知，卵爪卵骨卵母细胞在细胞核中累积丝状或F-肌动蛋白，但目前未知F-Actin是否还在胚胎核中积累。使用荧光标记的肌动蛋白记者，我们检查了胚胎胚胎细胞中的肌动蛋白分布，发现F-actin在Blastula阶段期间核心累积，但在胃肠杆菌期间没有。为了进一步调查核F-actin，我们设计了一种异爪蛋蛋蛋，可再现核心的核的形成。使用这种提取物，我们发现F-actin主要在亚核膜区域累积，并且对于将染色质与核包膜保持在发育良好的细胞核中是必要的。我们还提供了证据，核F-actin增加了核的结构稳定性，并有助于在以下M相的染色体上对准。这些结果表明核F-肌动蛋白积累在快速分开的大外爪疮细胞中的生理重要性。

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