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首页> 外文期刊>Genes to cells : >In vitro site-specific recombination mediated by the tyrosine recombinase XerA of Thermoplasma acidophilum
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In vitro site-specific recombination mediated by the tyrosine recombinase XerA of Thermoplasma acidophilum

机译：在酪氨酸重组酶XIA的体外特异性重组XIA的热固体酸嗜酸酯

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摘要

In organisms with circular chromosomes, such as bacteria and archaea, an odd number of homologous recombination events can generate a chromosome dimer. Such chromosome dimers cannot be segregated unless they are converted to monomers before cell division. In Escherichia coli, dimer-to-monomer conversion is mediated by the paralogous XerC and XerD recombinases at a specific dif site in the replication termination region. Dimer resolution requires the highly conserved cell division protein/chromosome translocase FtsK, and this site-specific chromosome resolution system is present or predicted in most bacteria. However, most archaea have only XerA, a homologue of the bacterial XerC/D proteins, but no homologues of FtsK. In addition, the molecular mechanism of XerA-mediated chromosome resolution in archaea has been less thoroughly elucidated than those of the corresponding bacterial systems. In this study, we identified two XerA-binding sites (dif1 and dif2) in the Thermoplasma acidophilum chromosome. In vitro site-specific recombination assays showed that dif2, but not dif1, serves as a target site for XerA-mediated chromosome resolution. Mutational analysis indicated that not only the core consensus sequence of dif2, but also its flanking regions play important roles in the recognition and recombination reactions mediated by XerA.
机译：在具有圆形染色体的生物体中，例如细菌和古痤疮，奇数的同源重组事件可以产生染色体二聚体。除非它们在细胞分裂之前转化为单体，否则不能偏析这种染色体二聚体。在大肠杆菌中，二聚体对单体转化率在复制终止区域的特定差距下由副骨Xerc和Xerd重组酶介导。二聚体分辨率需要高度保守的细胞分裂蛋白/染色体译团酶FTSK，并且在大多数细菌中存在或预测该位点特异性染色体分辨率系统。然而，大多数古代古老的XARA是细菌Xerc / D蛋白的同源物，但没有FTSK的同源物。此外，XA区域介导的染色体分辨率在古痤疮中的分子机制比相应的细菌系统的透明待彻底待彻底。在这项研究中，我们在热量嗜酸体染色体中鉴定了两个XEA结合位点（DIF1和DIF2）。体外特异性的重组测定表明，DIF2但不是DIF1，用作XERA介导的染色体分辨率的靶位点。突变分析表明，不仅核心共识序列，而且其侧翼区域也在X相介导的识别和重组反应中起重要作用。

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