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首页> 外文期刊>Genes to cells : >Accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells
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Accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells

机译：肌苷-II光谱复合物的积累在SRC转化的上皮细胞的顶端挤出中起着积极作用

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摘要

At the initial stage of carcinogenesis, transformation occurs in single cells within the epithelium. Recent studies have revealed that the newly emerging transformed cells are often apically eliminated from epithelial tissues. However, the underlying molecular mechanisms of this cancer preventive phenomenon still remain elusive. In this study, we first demonstrate that myosin-II accumulates in Src-transformed cells when they are surrounded by normal epithelial cells. Knock-down of the heavy chains of myosin-II substantially diminishes apical extrusion of Src cells, suggesting that accumulated myosin-II positively regulates the apical elimination of transformed cells. Furthermore, we have identified beta-spectrin as a myosin-II-binding protein under the coculture of normal and Src-transformed epithelial cells. beta-spectrin is also accumulated in Src cells that are surrounded by normal cells, and the beta-spectrin accumulation is regulated by myosin-II. Moreover, knock-down of beta-spectrin significantly suppresses apical extrusion of Src cells. Collectively, these results indicate that accumulation of the myosin-II-spectrin complex plays a positive role in apical extrusion of Src-transformed epithelial cells. Further elucidation of the molecular mechanisms of apical extrusion would lead to the establishment of a novel type of cancer preventive medicine.
机译：在癌发生的初始阶段，在上皮内的单细胞中发生转化。最近的研究表明，新出现的转化细胞通常从上皮组织中消除。然而，这种癌症预防现象的潜在分子机制仍然难以难以捉摸。在这项研究中，我们首先表明，当它们被正常上皮细胞包围时，肌霉素-II在SRC转化细胞中积聚。肌苷-II的重链的敲击基本上减少了SRC细胞的顶端挤出，表明积累的肌蛋白-II积极调节转化细胞的顶端消除。此外，我们在正常和Src转化的上皮细胞的共培养下鉴定了β-光谱作为肌霉素-II结合蛋白。 β光谱也累积在由正常细胞包围的SRC细胞中，并且β-光谱累积由肌球蛋白-II调节。此外，β光谱的敲低显着抑制了SRC细胞的顶端挤出。总的来说，这些结果表明，霉菌素-II光谱复合体的积累在SRC转化的上皮细胞的顶端挤出中起着积极作用。进一步阐明顶端挤出的分子机制将导致建立一种新型癌症预防医学。

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  • 来源
    《Genes to cells :》 |2018年第11期|共8页
  • 作者

    Takagi Mikio; Ikegawa Masaya; Shimada Takashi; Ishikawa Susumu; Kajita Mihoko; Maruyama Takeshi; Kamasaki Tomoko; Fujita Yasuyuki;

  • 作者单位

    Hokkaido Univ Grad Sch Chem Sci &

    Engn Inst Genet Med Div Mol Oncol Sapporo Hokkaido Japan;

    Doshisha Univ Fac Life &

    Med Sci Dept Life &

    Med Syst Genom Prote &

    Biomed Funct Kyoto Japan;

    Shimadzu Sci Instruments Innovat Ctr SHIMADZU Biosci Res Partnership Bothell WA USA;

    Hokkaido Univ Grad Sch Chem Sci &

    Engn Inst Genet Med Div Mol Oncol Sapporo Hokkaido Japan;

    Hokkaido Univ Grad Sch Chem Sci &

    Engn Inst Genet Med Div Mol Oncol Sapporo Hokkaido Japan;

    Hokkaido Univ Grad Sch Chem Sci &

    Engn Inst Genet Med Div Mol Oncol Sapporo Hokkaido Japan;

    Hokkaido Univ Grad Sch Chem Sci &

    Engn Inst Genet Med Div Mol Oncol Sapporo Hokkaido Japan;

    Hokkaido Univ Grad Sch Chem Sci &

    Engn Inst Genet Med Div Mol Oncol Sapporo Hokkaido Japan;

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  • 正文语种 eng
  • 中图分类 医学遗传学;
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