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Identification of novel phasiRNAs loci on long non-coding RNAs in Arabidopsis thaliana

机译：鉴定拟南芥长期非编码RNA的新型Phasirnas基因座

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摘要

Long non-coding RNAs (lncRNAs) are the "dark matters"involved in gene regulation with complex mechanisms. However, the functions of most lncRNAs remain to be determined. Our previous work revealed a massive number of degradome-supported cleavage signatures on Arabidopsis lncRNAs. Some of them have been confirmed associated with miRNAs-like sRNAs production, while others without long stem structure remain unexplored. A systematical search for phasiRNAs generating ability of these lncRNAs was conducted. Eight novel small RNA triggered lncRNA-phasiRNA pathways were discovered and three of them were found to be conserved in Arabidopsis, Oryza saliva, Glycine max and Gossypium hirsutum. Besides, Five novel ta-siRNAs derived from these lncRNAs were further identified to be involved in the regulation of plant development, stress responses and aromatic amino acids synthesis. These results substantially expanded the gene regulation mechanisms of lncRNAs.
机译：长期非编码RNA（LNCRNA）是具有复杂机制的基因调控中涉及的“黑暗事项”。 但是，大多数LNCRNA的功能仍然确定。 我们以前的工作揭示了拟南芥LNCRNA上的大量降低的可降低的裂解签名。 其中一些已被确认与MiRNA类似的SRNA生产相关，而没有长茎结构的其他人仍未探索。 进行了对这些LNCRNA的phasirnas产生能力的系统搜索。 发现了八种新型的小RNA触发的LNCRNA-PhasiRNA途径，发现其中三种是拟南芥，Oryza Saliva，甘氨酸最大和Gossypium Hirsutum保守。 此外，进一步鉴定了来自这些LNCRNA的五种新的TA-SIRNA，参与了植物发育，应力反应和芳族氨基酸合成的调节。 这些结果基本上扩展了LNCRNA的基因调控机制。

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