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SRNAome and transcriptome analysis provide insight into strawberry fruit ripening

机译:SRNAMEOM和转录组分析提供了对草莓果实成熟的洞察力

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摘要

Strawberry fruit ripening is a complex process affected by multiple factors at different regulation levels. To elucidate the regulation mechanisms, the combined analysis of sRNAome and transcriptome were used. A total of 124 known and 190 novel miRNAs were found, 62 of them were significantly differentially expressed (DE). The targets of the DE miRNAs were parsed and several TFs, such as SPL, ARF, WRKY, and TCP, were found to be involved in ripening. Elevated CO 2 can significantly postpone ripening and miR156, miR166f, miR171a, and miR171d were the DE miRNAs. Transcriptome analysis found 313 DE genes related to fruit ripening, including cell wall metabolism-related genes, color-related genes, ethylene-related genes, and genes encoding TFs such as MYB, SPL, NAC, TCP, and ARF. Based on above, a combined regulatory model involved in fruit ripening was created. These results provide valuable information for understanding the complicated coordinated regulatory network of strawberry fruit ripening.
机译:草莓果实成熟是一种复杂的过程,受不同调控水平的多种因素影响。为了阐明调节机制,使用了SRNA组和转录组的组合分析。发现总共124名和190个新的miRNA,其中62种显着表达(DE)。解析了De miRNA的靶标,发现了几种TFS,例如SPL,ARF,WRKY和TCP,涉及成熟。升高的CO 2可以显着推迟成熟,miR156,mir166f,miR171a和mir171d是de mirnas。转录组分析发现与果实熟化有关的313个基因,包括细胞壁代谢相关基因,颜色相关基因,相关基因和编码TF的基因,如MYB,SPL,NAC,TCP和ARF。基于以上,创建了果实成熟的组合调控模式。这些结果提供了理解草莓果实成熟的复杂协调监管网络的宝贵信息。

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