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首页> 外文期刊>Genomics >Gene-centric metegenome analysis reveals diversity of Pseudomonas aeruginosa biofilm gene orthologs in fresh water ecosystem
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Gene-centric metegenome analysis reveals diversity of Pseudomonas aeruginosa biofilm gene orthologs in fresh water ecosystem

机译：基因中心的聚蛋白酶分析揭示了淡水生态系统中假单胞菌铜绿假单胞菌的多样性

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摘要

Metagenomic analysis of biofilm forming bacteria in environmental samples remains challenging due to the non-availability of gene sequences of most of the uncultivable bacteria. Sequences of Pseudomonas aeruginosa PAO1-UW genes involved either directly or indirectly in biofilm formation were analyzed using BLASTn to obtain matching sequences from different strain, species and genus. Conserved regions in the functional domain of the amino acid sequences were used to design common primers for direct PCR analysis of freshwater metagenomes. Seven key genes such as aceA, clpP, typA, cbrA, phoR, rpoS and gacA involved in biofilm formation were validated. The ortholog genes belonged to wide range of Pseudomonas sp. indicating the diversity of biofilm genes and the conservation of protein functional domains. The approach would also help in analyzing the expression of biofilm genes in different bacteria of freshwater systems for monitoring toxic contaminations such as organic or inorganic pollutants.
机译：由于大多数未掩露细菌的基因序列的非可用性，生物膜形成细菌的聚蛋白分析仍然挑战。使用BLASTN分析直接或间接地在生物膜形成中涉及的假单胞菌铜绿素Pao1-UW基因，得到来自不同菌株，物种和属的匹配序列。氨基酸序列的功能结构域中的保守区域用于设计淡水偏心组的直接PCR分析的常见引物。验证了七个关键基因，如acea，clpp，典型的粘合剂，涉及生物膜形成中涉及的生物膜形成。地下基因属于广泛的假鼠SP。表明生物膜基因的多样性以及蛋白质功能域的保护。该方法还将有助于分析淡水系统不同细菌中生物膜基因的表达，用于监测有机或无机污染物等毒性污染物。

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