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Characterization and function of the T-box 1 gene in Chinese giant salamander Andrias davidianus

机译：中国巨型蝾螈andrias davidianus的T字幕1基因的表征与功能

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摘要

We characterized the Andrias davidianus T-box 1 (Tbx1) gene. Tbx1 expression was high in testis and low in other examined tissues. Immunohistochemistry detected tbx1 expression in somatic and germ cells 62 days post-hatching (dph), prior to gonad differentiation. At 210 dph, after gonad differentiation, tbx1 was expressed in spermatogonia and testis somatic cells and in granulosa cells in ovary. Tbx1 expression was up-regulated in ovary after high temperature treatment. In the neomale, tbx1 expression showed a similar profile to normal males, and vice-versa for genetic male. Over-expression of tbx1 in females after injection of TBX1 protein downregulated the female-biased genes cyp19a and foxl2 and up-regulated the male-biased amh gene. When tbx1 was knocked down by tbx1/siRNA, cyp19a and foxl2 expression was up-regulated, and expression of amh, cyp26a, dmrt1, and wt1 was down-regulated. Results suggest that tbx1 influenced sex-related gene expression and participates in regulation of A. davidianus testis development.
机译：我们以andrias davidianus t盒1（tbx1）基因为特征。 TBX1表达在睾丸中高，在其他检查组织中较低。免疫组织化学检测到体细胞和胚芽细胞中的TBX1表达62天在阴唇分化之前（DPH）。在Gonad分化之后，TBX1在精子寄生和睾丸细胞和卵巢中的颗粒细胞中表达。高温处理后，TBX1表达上调卵巢。在NeoMale中，TBX1表达显示出与正常雄性相似的概况，对遗传男性反之亦然。注射Tbx1蛋白后的女性的TBX1的过度表达下调了雌性偏置的基因CYP19A和FOXL2并上调了雄性偏置的AMH基因。当TBX1通过TBX1 / siRNA被敲击时，上调CYP19A和FOXL2表达，并对AMH，CYP26A，DMRT1和WT1的表达进行下调。结果表明，TBX1影响了性与性基因表达，并参与了A. Davidianus Testis开发的调节。

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