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Identification and expression analysis of miRNA in hybrid snakehead by deep sequencing approach and their targets prediction

机译：深度测序方法及其靶预测杂交蛇头miRNA鉴定及表达分析及其目标预测

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摘要

MicroRNAs (miRNAs) play important regulatory roles in numerous biological processes, but there is no report on miRNAs of hybrid snakehead. In this study, four independent small RNA libraries were constructed from the spleen, liver kidney and muscle of hybrid snakehead. These libraries were sequenced using deep sequencing technology, as result, a total of 1,067,172, 1,152,002, 1,653,941 and 970,866 clean reads from these four libraries were obtained. 252 known miRNAs and 63 putative novel miRNAs in these small RNA dataset were identified. The stem-loop RT-qPCR analysis indicated that eight known miRNAs and two novel miRNAs show different expression in eight different kinds of tissues. For better understanding the functions of miRNAs, 95,947 predicated target genes were analyzed by GO and their pathways, the results indicated that these targets of the identified miRNAs are involved in a broad range of physiological functions.
机译：MicroRNA（miRNA）在许多生物过程中起着重要的监管作用，但没有关于杂交蛇头的miRNA的报道。 在这项研究中，由脾脏，肝肾和杂交蛇头的肌肉构建了四种独立的小RNA文库。 使用深度测序技术对这些文库进行测序，结果，总共1,067,172,1,152,002,1,653,941和970,866获得了这四个文库的清洁读数。 鉴定了252名已知的miRNA和63个推定的新型miRNA在这些小RNA数据集中。 茎环RT-QPCR分析表明，八个已知的miRNA和两种新的miRNA在八种不同组织中显示出不同的表达。 为了更好地理解MiRNA的功能，通过GO及其途径分析95,947个探测目标基因，结果表明所识别的miRNA的这些靶标在广泛的生理功能中。

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