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Differential gene expression of Plasmodium homocircumflexum (lineage pCOLL4) across two experimentally infected passerine bird species

机译:两种实验感染脊髓鸟类疟原虫同源疟原虫(谱系PCOLL4)的差异基因表达

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摘要

Plasmodium parasites are present in a wide range of host species, some of which tend to be more susceptible than others, potentially as an outcome of evolved tolerance or resistance. Common starlings seem to cope with malaria infection while common crossbills are more susceptible to the same infections. That raises the question if the parasites rely on the same molecular mechanisms regardless of host species or do Plasmodium parasites change gene-expressions in accordance to the environment different hosts might provide? We used RNA-sequencing from starlings and crossbills, experimentally infected with Plasmodium homocircumflexum (lineage pCOLL4). The assembled transcriptome contained a total of 26,733 contigs. Parasite expression patterns differed between bird species. Parasites had higher expression of cell-invasion genes when infecting crossbills compared to starlings whereas in starlings genes related to apoptosis or/and oxidative stress showed higher expression levels. This article reveals how a Plasmodium parasite might adjust its expression and gene function depending on the host species infected.
机译:疟原虫存在于各种宿主物种中,其中一些往往比其他宿主物种更容易受到更容易受到影响。可能是耐受性或抗性的结果。常见的椋鸟似乎应对疟疾感染,而常见的横纤牌更容易受到同样的感染。如果寄生虫依赖于相同的分子机制,无论宿主物种或寄生虫寄生虫更换疟原虫,根据环境不同的宿主可能提供吗?我们使用椋鸟和十字架的RNA测序,实验感染疟原虫(谱系PCOLL4)。组装的转录组含有总共26,733个葡萄片。寄生虫表达模式不同于鸟类。当与饥饿相比,寄生虫表达细胞侵入基因的表达更高,而与凋亡或/和氧化应激相关的辛酸基因显示出更高的表达水平。本文揭示了寄生虫疟原虫如何根据感染的宿主物种调整其表达和基因功能。

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    《Genomics》 |2020年第4期|共9页
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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 医学遗传学;
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