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首页> 外文期刊>Genes and genomics >Analysis of differential gene expression in cold-tolerant vs. cold-sensitive varieties of snap bean (Phaseolus vulgaris L.) in response to low temperature stress
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Analysis of differential gene expression in cold-tolerant vs. cold-sensitive varieties of snap bean (Phaseolus vulgaris L.) in response to low temperature stress

机译：响应于低温胁迫的冷耐耐寒性与冷敏品种的差分基因表达分析

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摘要

Background Snap bean, Phaseolus vulgaris L., as a warm-season vegetable, low temperature stress seriously affect the yield and quality. At present, little is known about the genes and molecular regulation mechanism in cold response in snap bean exposed to low temperature. Objectives Our objectives were to identify the low temperature response genes in snap bean and to examine differences in the gene response between cold-tolerant and cold-sensitive genotypes. Methods We used two highly inbred snap bean lines in this study, the cold-tolerant line '120', and the cold-sensitive line '093'. The plants were grown to the three leaf and one heart stage and exposed to 4 degrees C low temperature. We used RNA sequencing (RNA-seq) to analyze the differences of gene expression. Results 988 and 874 cold-responsive genes were identified in 'T120 vs CK120' and 'T093 vs CK093' ('T' stands for low temperature treatment, and 'CK' stands for control at room temperature), respectively. Of these, 555 and 442 genes were unique to cold-stressed lines '120' and '093', respectively compared to the control. Our analysis of these differentially expressed genes indicates that Ca2+, ROS, and hormones act as signaling molecules that play important roles in low temperature response in P. vulgaris. Altering the expression of genes in these signaling pathways activates expression of downstream response genes which can interact with other signaling regulatory networks. This may maintained the balance of ROS and hormones, making line '120' more cold-tolerant than line '093'. Conclusion Our results provide a preliminarily understanding of the molecular basis of low temperature response in snap bean, and also establish a foundation for the future genetic improvement of cold sensitivity in snap bean by incorporating genes for cold tolerance.
机译：背景豌豆，Phaseolus寻常L.，作为暖季蔬菜，低温压力严重影响产量和质量。目前，在暴露于低温的卡纸中的冷响应中的基因和分子调控机制很少。目的我们的目标是鉴定Snap Bean中的低温响应基因，并检查耐冷耐寒性和冷敏基因型之间基因响应的差异。方法我们在本研究中使用了两条高度近交豆类线，冷却线“120”和冷敏线“093”。将植物生长到三片叶和一个心脏阶段，暴露于4℃的低温。我们使用RNA测序（RNA-SEQ）来分析基因表达的差异。结果988和874在“T120 VS CK120”中鉴定了冷敏敏基因，“T093 VS CK093”（'T'代表低温处理，分别为“CK”在室温下控制）。其中，555和442个基因分别与控制相比，冷应力线'120'和'093'是独一无二的。我们对这些差异表达基因的分析表明CA2 +，ROS和激素作为发挥重要作用的信号分子，其在寻常的低温响应中起重要作用。改变这些信号传导途径中基因的表达激活可以与其他信号传导网络相互作用的下游响应基因的表达。这可能会保持ROS和激素的平衡，使得线120'更冷的耐冷比线'093'。结论我们的结果对快乐豆类的低温响应的分子基础提供了初步了解，并通过掺入冷耐耐力基因来建立未来冷豆类冷敏感性的遗传改善的基础。

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  • 来源
    《Genes and genomics》 |2019年第12期|共11页
  • 作者

    Liu Chang; Yang Xiaoxu; Yan Zhishan; Fan Youjun; Feng Guojun; Liu Dajun;

  • 作者单位

    Heilongjiang Univ Acad Crop Sci Hort Dept 74 Xuefu Rd Harbin 150000 Heilongjiang Peoples R China;

    Heilongjiang Univ Acad Crop Sci Hort Dept 74 Xuefu Rd Harbin 150000 Heilongjiang Peoples R China;

    Heilongjiang Univ Acad Crop Sci Hort Dept 74 Xuefu Rd Harbin 150000 Heilongjiang Peoples R China;

    Heilongjiang Univ Acad Crop Sci Hort Dept 74 Xuefu Rd Harbin 150000 Heilongjiang Peoples R China;

    Heilongjiang Univ Acad Crop Sci Hort Dept 74 Xuefu Rd Harbin 150000 Heilongjiang Peoples R China;

    Heilongjiang Univ Acad Crop Sci Hort Dept 74 Xuefu Rd Harbin 150000 Heilongjiang Peoples R China;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 生物科学;
  • 关键词

    Snap bean; Low temperature stress; Gene expression; RNA-seq;

    机译：Snap Bean;低温胁迫;基因表达;RNA-SEQ;

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