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首页> 外文期刊>FEMS Microbiology Ecology >High-throughput sequencing revealed novel Dehalococcoidia in dechlorinating microbial enrichments from PCB-contaminated marine sediments
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High-throughput sequencing revealed novel Dehalococcoidia in dechlorinating microbial enrichments from PCB-contaminated marine sediments

机译：高通量测序揭示了来自PCB污染的海洋沉积物的脱氯微生物富集的新型脱卤素

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In this study, six PCE-to-ethene dechlorinating cultures, fed with a fermentable substrate (lactate) or hydrogen as electron donor, were obtained from PCB and PCE dechlorinating microcosms constructed with PCB-contaminated marine sediments. A novel Chloroflexi member (OTU-DIS1) affiliated to Dehalococcoidales Incertae Sedis, only distantly related to known dechlorinating bacteria, dominated the enrichment cultures (up to 86% of total OTUs). Sulfate-, thiosulfate- and sulfur-reducing bacteria affiliated to genera Desulfobacter, Dethiosulfatibacter and Desulfuromusa were also found to lesser extent. Remarkably, tceA, vcrA and the bifunctional PCE/PCB dehalogenase genes pcbA1, pcbA4 and pcbA5 were found in all dechlorinating microbial enrichments indicating the coexistence of different Dehalococcoides mccartyi strains. The reductive dechlorination rate in each culture remained unvaried over long-term operation (approximate to 30 months) and ranged between 0.85 and 0.97 mmol Cl-1 released L-1 d(-1) in the lactate-fed microbial enrichments and between 0.66 and 0.85 mmol Cl-1 released L-1 d(-1) in the H-2-fed microbial enrichments. Overall, this study highlights the presence of yet unexplored biodiversity in PCBs contaminated marine sediments and indicates these environments as promising sources of novel organohalide-respiring bacteria.
机译：在该研究中，用PCB和PCE脱氯微观的PCB污染的海洋沉积物构建的PCB和PCE脱氯薄膜中获得六种PCE-乙烯脱氯培养物或作为电子供体。一种新的氯昔上会员（Otu-Dis1）隶属于去卤化物瘢痕疙瘩，仅与已知的脱氯化菌细菌居住，将富集培养物（占总OTU的86％）占主导地位。在较小程度上发现，硫酸盐 - ，硫代硫酸盐和还原细菌，DethioSulfatibacter和脱硫血清患者。在所有脱氯的微生物富集中发现，发现TCEA，VCRA和双官能PCE / PCB脱氢酶基因PCBA1，PCBA4和PCBA5，表明不同除去的微生物症麦卡替西菌株的共存。每种培养中的还原脱氯率保持不变，在长期操作（近似为30个月），在乳酸喂养的微生物富集中释放0.85和0.97mmol Cl-1释放的L-1 d（-1）和0.66和0.66 0.85mmol Cl-1在H-2喂养的微生物富集中释放L-1 D（-1）。总体而言，这项研究突出了PCBS受污染的海洋沉积物中尚未开发的生物多样性的存在，并表明这些环境作为新型有机卤化物呼吸细菌的有前途的来源。

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