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首页> 外文期刊>FEMS Microbiology Letters >Genomic characterization of Vibrio parahaemolyticus from Pacific white shrimp and rearing water in Malaysia reveals novel sequence types and structural variation in genomic regions containing the Photorhabdus insect-related (Pir) toxin-like genes
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Genomic characterization of Vibrio parahaemolyticus from Pacific white shrimp and rearing water in Malaysia reveals novel sequence types and structural variation in genomic regions containing the Photorhabdus insect-related (Pir) toxin-like genes

机译：马来西亚太平洋白虾和饲养水的弧菌副溶血性的基因组特征揭示了一种基因组区域的新序列类型和结构变异，含有含有光炎昆虫相关（PIR）毒素的基因的基因组区域

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摘要

The Malaysian and global shrimp aquaculture production has been significantly impacted by acute hepatopancreatic necrosis disease (AHPND) typically caused by Vibrio parahaemolyticus harboring the pVA plasmid containing the pirA(Vp) and pirB(Vp) genes, which code for Photorhabdus insect-related (Pir) toxin. The limited genomic resource for V. parahaemolyticus strains from Malaysian aquaculture farms precludes an in-depth understanding of their diversity and evolutionary relationships. In this study, we isolated shrimp-associated and environmental (rearing water) V. parahaemolyticus from three aquaculture farms located in Northern and Central Malaysia followed by whole-genome sequencing of 40 randomly selected isolates on the Illumina MiSeq. Phylogenomic analysis and multilocus sequence typing (MLST) reveal distinct lineages of V. parahaemolyticus that harbor the pirAB(Vp) genes. The recovery of pVA plasmid backbone devoid of pirA(Vp) or pirAB(Vp) in some V. parahaemolyticus isolates suggests that the toxin genes are prone to deletion. The new insight gained from phylogenomic analysis of Asian V. parahaemolyticus, in addition to the observed genomic instability of pVa plasmid, will have implications for improvements in aquaculture practices to diagnose, treat or limit the impacts of this disease.
机译：马来西亚和全球虾水产养殖产量受到急性肝病坏死疾病（AHPND）的显着影响，通常由含有Pira（vp）和PiRB（vp）基因的PVA质粒，该抗PVA质粒引起的vibrio副溶血性，该昆虫与昆虫相关（PIR ）毒素。来自马来西亚水产养殖农场的V.Vahaemolyticus菌株的有限基因组资源排除了对其多样性和进化关系的深入了解。在这项研究中，我们从位于马来西亚北部和马来西亚北部的三个水产养殖场的虾相关和环境（饲养水）V. Parahaemolyticus。在Illumina Miseq上的40个随机选择的分离物的全基因组测序。系统核发生组学分析和多层序列键入（MLST）揭示了患有皮拉采购（VP）基因的V.Varahaemolyticus的不同谱系。在一些V.乙酰溶血性分离物中缺乏Pira（vp）或pirab（vp）的PVA质粒骨架的回收表明毒素基因易于缺失。来自亚洲V.乙酰灭菌剂的系统核发科学分析中获得的新洞察力，除了观察到PVA质粒的基因组不稳定性外，还对水产养殖实践的改进有影响，以诊断，治疗或限制该疾病的影响。

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